Table 1

Genes identified in this study and their predicted products

New gad genesPredicted products
acp-2Acid phosphatase
apy-1Apyrase
gad-2 (C10A4.5)Transmembrane protein
gad-3 (B0222.9)Xanthine dehydrogenase
glo-3Gut granule/lysosome formation, expressed in early E lineage
kin-33Kinase
sdz-6Unknown
sdz-19Unknown
sdz-22Transthyretin family
sdz-27Unknown
sdz-28BTB/POZ domain, MATH domain
sdz-31Unknown, expressed in early E and MS lineages
sdz-36Unknown
tbx-11T-box transcription factor
ugt-23UDP-glucuronosyl transferase
vet-6Very early transcript, contains a spectrin repeat
New gadr genes
alh-13Dehydrogenase/reductase
drr-1Dietary restriction response
F44A2.7Unknown
fbxb-19F-box protein
fbxb-35F-box protein
fbxb-38F-box protein
gadr-1 (F58D2.1)ZYG-11-like protein, possible SRS for a CUL-2 ubiquitin ligase
gadr-2 (C33A12.12)ZYG-11-like protein, possible SRS for a CUL-2 ubiquitin ligase
gadr-3 (F47D12.5)ZYG-11-like protein, possible SRS for a CUL-2 ubiquitin ligase
gadr-4 (W06A11.2)ZYG-11-like protein, possible SRS for a CUL-2 ubiquitin ligase
gadr-5 (Y71A12B.17)ZYG-11-like protein, possible SRS for a CUL-2 ubiquitin ligase
gadr-6 (F47G4.2)ZYG-11-like protein, possible SRS for a CUL-2 ubiquitin ligase
prx-5Peroxisome import
sdz-18BTB/POZ domain, MATH domain
sdz-21Unknown
sdz-23Transmembrane, EGF domain, expressed in early E lineage
sdz-32Unknown
sdz-34Predicted E3 ubiquitin ligase
  • Listed are 16 new gad genes (gastrulation-defective: genes whose loss of function results in gastrulation phenotypes) and 18 new gadr genes (gastrulation defective, redundant: genes whose loss of function only results in gastrulation phenotypes in combination with loss of function of other genes). Pend-1::GFP expression was examined for a small number of embryos after RNAi of all gad genes except gad-3, sdz-19, sdz-22, tbx-11, and was seen to be low or absent for 1/1 acp-2(RNAi) embryo, 1/1 ugt-23(RNAi) embryo, and 2/6 gad-2(RNAi) embryos, suggesting that these genes may affect endoderm specification as well. Genes given new names here are listed as gad- or gadr-, with the corresponding sequence name in parentheses. Expression data are from Rabbitts et al. 2008 for glo-3, and Robertson et al. 2004 for sdz genes.