TABLE 2

Recovery of alleles at the same locus: estimation of the minimum number of zym gene loci

Search ID No.MutagenNew locia/total lociEstimated no. zym locib
1-80EMS1/1
2-82UV6/74
3-83UV9/9
4-84UV6/6
5-84FdURD2/2
6-85FdURD2/438
7-86UV0/119
8-87, 9-87FdURD2/547
10-88UV4/749
11-92; 12-92EMS, MMS15/2288
13-95MMS5/1179
  • a Although the sum of the new loci identified is 52, mutants representing only 46 of these loci have been retained. Mutants showing extremely low mating efficiencies or an excessively leaky phenotype have been discarded.

  • b The number of zym loci is calculated from the formula n = t/a where t is the number of test crosses performed (number of newly isolated complementing mutants × number of mutants presently in the collection) and a is the number of cases of noncomplementation in the test crosses.