TABLE 1

Summary of recovery of zym following induced mutagenesis

Search ID no.StrainMutagenNo. clones analyzedNo. zym mutantsNo. gene lociaMutation frequencyb
1-80cspr-fd1EMS2500512 × 10−3
2-82dWT15cUV12001478.3 × 10−3
3-83UTEX220UV87889e1 × 10−2
4-84UTEX220UV872666.9 × 10−3
5-84spf-fd1FdURDNA22NA
6-85WT15cFdURD415541 × 10−2
7-86UTEX220UV168215.9 × 10−3
8-87WT15cFdURD600323.3 × 10−3
9-87UTEX220FdURD500336 × 10−3
10-88UTEX220UV1219876.6 × 10−3
11-92WT15cEMS22971183 × 10−3
12-92WT15cMMS22231896.3 × 10−3
13-95UTEX220MMS322414113.7 × 10−3
  • NA, data not available.

  • a The number of gene loci is assumed to be equal to the number of complementation groups.

  • b Mutation frequencies are based on the number of zym mutants recovered that complement one another and any noncomplementing mutants that are known to have arisen independently of one another. Noncomplementing mutants that cannot be assumed to have arisen independently are assumed to be mitotic daughters produced during postmutagenesis growth (see materials and methods).

  • c VanWinkle-Swift and Bauer (1982).

  • d VanWinkle-Swift and Burrascano (1983).

  • e One mutant derived from this search was later found to be carrying two unlinked zym mutations (Parmelee 1983).