RT Journal Article SR Electronic T1 The Transgenic RNAi Project at Harvard Medical School: Resources and Validation JF Genetics JO Genetics FD Genetics Society of America SP 843 OP 852 DO 10.1534/genetics.115.180208 VO 201 IS 3 A1 Perkins, Lizabeth A. A1 Holderbaum, Laura A1 Tao, Rong A1 Hu, Yanhui A1 Sopko, Richelle A1 McCall, Kim A1 Yang-Zhou, Donghui A1 Flockhart, Ian A1 Binari, Richard A1 Shim, Hye-Seok A1 Miller, Audrey A1 Housden, Amy A1 Foos, Marianna A1 Randkelv, Sakara A1 Kelley, Colleen A1 Namgyal, Pema A1 Villalta, Christians A1 Liu, Lu-Ping A1 Jiang, Xia A1 Huan-Huan, Qiao A1 Wang, Xia A1 Fujiyama, Asao A1 Toyoda, Atsushi A1 Ayers, Kathleen A1 Blum, Allison A1 Czech, Benjamin A1 Neumuller, Ralph A1 Yan, Dong A1 Cavallaro, Amanda A1 Hibbard, Karen A1 Hall, Don A1 Cooley, Lynn A1 Hannon, Gregory J. A1 Lehmann, Ruth A1 Parks, Annette A1 Mohr, Stephanie E. A1 Ueda, Ryu A1 Kondo, Shu A1 Ni, Jian-Quan A1 Perrimon, Norbert YR 2015 UL http://www.genetics.org/content/201/3/843.abstract AB To facilitate large-scale functional studies in Drosophila, the Drosophila Transgenic RNAi Project (TRiP) at Harvard Medical School (HMS) was established along with several goals: developing efficient vectors for RNAi that work in all tissues, generating a genome-scale collection of RNAi stocks with input from the community, distributing the lines as they are generated through existing stock centers, validating as many lines as possible using RT–qPCR and phenotypic analyses, and developing tools and web resources for identifying RNAi lines and retrieving existing information on their quality. With these goals in mind, here we describe in detail the various tools we developed and the status of the collection, which is currently composed of 11,491 lines and covering 71% of Drosophila genes. Data on the characterization of the lines either by RT–qPCR or phenotype is available on a dedicated website, the RNAi Stock Validation and Phenotypes Project (RSVP, http://www.flyrnai.org/RSVP.html), and stocks are available from three stock centers, the Bloomington Drosophila Stock Center (United States), National Institute of Genetics (Japan), and TsingHua Fly Center (China).