RT Journal Article
SR Electronic
T1 The Transgenic RNAi Project at Harvard Medical School: Resources and Validation
JF Genetics
JO Genetics
FD Genetics Society of America
SP 843
OP 852
DO 10.1534/genetics.115.180208
VO 201
IS 3
A1 Perkins, Lizabeth A.
A1 Holderbaum, Laura
A1 Tao, Rong
A1 Hu, Yanhui
A1 Sopko, Richelle
A1 McCall, Kim
A1 Yang-Zhou, Donghui
A1 Flockhart, Ian
A1 Binari, Richard
A1 Shim, Hye-Seok
A1 Miller, Audrey
A1 Housden, Amy
A1 Foos, Marianna
A1 Randkelv, Sakara
A1 Kelley, Colleen
A1 Namgyal, Pema
A1 Villalta, Christians
A1 Liu, Lu-Ping
A1 Jiang, Xia
A1 Huan-Huan, Qiao
A1 Wang, Xia
A1 Fujiyama, Asao
A1 Toyoda, Atsushi
A1 Ayers, Kathleen
A1 Blum, Allison
A1 Czech, Benjamin
A1 Neumuller, Ralph
A1 Yan, Dong
A1 Cavallaro, Amanda
A1 Hibbard, Karen
A1 Hall, Don
A1 Cooley, Lynn
A1 Hannon, Gregory J.
A1 Lehmann, Ruth
A1 Parks, Annette
A1 Mohr, Stephanie E.
A1 Ueda, Ryu
A1 Kondo, Shu
A1 Ni, Jian-Quan
A1 Perrimon, Norbert
YR 2015
UL http://www.genetics.org/content/201/3/843.abstract
AB To facilitate large-scale functional studies in Drosophila, the Drosophila Transgenic RNAi Project (TRiP) at Harvard Medical School (HMS) was established along with several goals: developing efficient vectors for RNAi that work in all tissues, generating a genome-scale collection of RNAi stocks with input from the community, distributing the lines as they are generated through existing stock centers, validating as many lines as possible using RT–qPCR and phenotypic analyses, and developing tools and web resources for identifying RNAi lines and retrieving existing information on their quality. With these goals in mind, here we describe in detail the various tools we developed and the status of the collection, which is currently composed of 11,491 lines and covering 71% of Drosophila genes. Data on the characterization of the lines either by RT–qPCR or phenotype is available on a dedicated website, the RNAi Stock Validation and Phenotypes Project (RSVP, http://www.flyrnai.org/RSVP.html), and stocks are available from three stock centers, the Bloomington Drosophila Stock Center (United States), National Institute of Genetics (Japan), and TsingHua Fly Center (China).