PT  - JOURNAL ARTICLE
AU  - Perkins, Lizabeth A.
AU  - Holderbaum, Laura
AU  - Tao, Rong
AU  - Hu, Yanhui
AU  - Sopko, Richelle
AU  - McCall, Kim
AU  - Yang-Zhou, Donghui
AU  - Flockhart, Ian
AU  - Binari, Richard
AU  - Shim, Hye-Seok
AU  - Miller, Audrey
AU  - Housden, Amy
AU  - Foos, Marianna
AU  - Randkelv, Sakara
AU  - Kelley, Colleen
AU  - Namgyal, Pema
AU  - Villalta, Christians
AU  - Liu, Lu-Ping
AU  - Jiang, Xia
AU  - Huan-Huan, Qiao
AU  - Wang, Xia
AU  - Fujiyama, Asao
AU  - Toyoda, Atsushi
AU  - Ayers, Kathleen
AU  - Blum, Allison
AU  - Czech, Benjamin
AU  - Neumuller, Ralph
AU  - Yan, Dong
AU  - Cavallaro, Amanda
AU  - Hibbard, Karen
AU  - Hall, Don
AU  - Cooley, Lynn
AU  - Hannon, Gregory J.
AU  - Lehmann, Ruth
AU  - Parks, Annette
AU  - Mohr, Stephanie E.
AU  - Ueda, Ryu
AU  - Kondo, Shu
AU  - Ni, Jian-Quan
AU  - Perrimon, Norbert
TI  - The Transgenic RNAi Project at Harvard Medical School: Resources and Validation
AID  - 10.1534/genetics.115.180208
DP  - 2015 Nov 01
TA  - Genetics
PG  - 843--852
VI  - 201
IP  - 3
4099  - http://www.genetics.org/content/201/3/843.short
4100  - http://www.genetics.org/content/201/3/843.full
SO  - Genetics2015 Nov 01; 201
AB  - To facilitate large-scale functional studies in Drosophila, the Drosophila Transgenic RNAi Project (TRiP) at Harvard Medical School (HMS) was established along with several goals: developing efficient vectors for RNAi that work in all tissues, generating a genome-scale collection of RNAi stocks with input from the community, distributing the lines as they are generated through existing stock centers, validating as many lines as possible using RT–qPCR and phenotypic analyses, and developing tools and web resources for identifying RNAi lines and retrieving existing information on their quality. With these goals in mind, here we describe in detail the various tools we developed and the status of the collection, which is currently composed of 11,491 lines and covering 71% of Drosophila genes. Data on the characterization of the lines either by RT–qPCR or phenotype is available on a dedicated website, the RNAi Stock Validation and Phenotypes Project (RSVP, http://www.flyrnai.org/RSVP.html), and stocks are available from three stock centers, the Bloomington Drosophila Stock Center (United States), National Institute of Genetics (Japan), and TsingHua Fly Center (China).