PT - JOURNAL ARTICLE AU - Perkins, Lizabeth A. AU - Holderbaum, Laura AU - Tao, Rong AU - Hu, Yanhui AU - Sopko, Richelle AU - McCall, Kim AU - Yang-Zhou, Donghui AU - Flockhart, Ian AU - Binari, Richard AU - Shim, Hye-Seok AU - Miller, Audrey AU - Housden, Amy AU - Foos, Marianna AU - Randkelv, Sakara AU - Kelley, Colleen AU - Namgyal, Pema AU - Villalta, Christians AU - Liu, Lu-Ping AU - Jiang, Xia AU - Huan-Huan, Qiao AU - Wang, Xia AU - Fujiyama, Asao AU - Toyoda, Atsushi AU - Ayers, Kathleen AU - Blum, Allison AU - Czech, Benjamin AU - Neumuller, Ralph AU - Yan, Dong AU - Cavallaro, Amanda AU - Hibbard, Karen AU - Hall, Don AU - Cooley, Lynn AU - Hannon, Gregory J. AU - Lehmann, Ruth AU - Parks, Annette AU - Mohr, Stephanie E. AU - Ueda, Ryu AU - Kondo, Shu AU - Ni, Jian-Quan AU - Perrimon, Norbert TI - The Transgenic RNAi Project at Harvard Medical School: Resources and Validation AID - 10.1534/genetics.115.180208 DP - 2015 Nov 01 TA - Genetics PG - 843--852 VI - 201 IP - 3 4099 - http://www.genetics.org/content/201/3/843.short 4100 - http://www.genetics.org/content/201/3/843.full SO - Genetics2015 Nov 01; 201 AB - To facilitate large-scale functional studies in Drosophila, the Drosophila Transgenic RNAi Project (TRiP) at Harvard Medical School (HMS) was established along with several goals: developing efficient vectors for RNAi that work in all tissues, generating a genome-scale collection of RNAi stocks with input from the community, distributing the lines as they are generated through existing stock centers, validating as many lines as possible using RT–qPCR and phenotypic analyses, and developing tools and web resources for identifying RNAi lines and retrieving existing information on their quality. With these goals in mind, here we describe in detail the various tools we developed and the status of the collection, which is currently composed of 11,491 lines and covering 71% of Drosophila genes. Data on the characterization of the lines either by RT–qPCR or phenotype is available on a dedicated website, the RNAi Stock Validation and Phenotypes Project (RSVP, http://www.flyrnai.org/RSVP.html), and stocks are available from three stock centers, the Bloomington Drosophila Stock Center (United States), National Institute of Genetics (Japan), and TsingHua Fly Center (China).