RT Journal Article
SR Electronic
T1 Predicting and Testing Physical Locations of Genetically Mapped Loci on Tomato Pachytene Chromosome <em>1</em>
JF Genetics
JO Genetics
FD Genetics Society of America
SP 2131
OP 2138
DO 10.1534/genetics.107.074138
VO 176
IS 4
A1 Chang, Song-Bin
A1 Anderson, Lorinda K.
A1 Sherman, Jamie D.
A1 Royer, Suzanne M.
A1 Stack, Stephen M.
YR 2007
UL http://www.genetics.org/content/176/4/2131.abstract
AB Predicting the chromosomal location of mapped markers has been difficult because linkage maps do not reveal differences in crossover frequencies along the physical structure of chromosomes. Here we combine a physical crossover map based on the distribution of recombination nodules (RNs) on Solanum lycopersicum (tomato) synaptonemal complex 1 with a molecular genetic linkage map from the interspecific hybrid S. lycopersicum × S. pennellii to predict the physical locations of 17 mapped loci on tomato pachytene chromosome 1. Except for one marker located in heterochromatin, the predicted locations agree well with the observed locations determined by fluorescence in situ hybridization. One advantage of this approach is that once the RN distribution has been determined, the chromosomal location of any mapped locus (current or future) can be predicted with a high level of confidence.