PT  - JOURNAL ARTICLE
AU  - Cheng, Mimi K.
AU  - Shearn, Allen
TI  - The Direct Interaction Between ASH2, a Drosophila Trithorax Group Protein, and SKTL, a Nuclear Phosphatidylinositol 4-Phosphate 5-Kinase, Implies a Role for Phosphatidylinositol 4,5-Bisphosphate in Maintaining Transcriptionally Active Chromatin
AID  - 10.1534/genetics.103.018721
DP  - 2004 Jul 01
TA  - Genetics
PG  - 1213--1223
VI  - 167
IP  - 3
4099  - http://www.genetics.org/content/167/3/1213.short
4100  - http://www.genetics.org/content/167/3/1213.full
SO  - Genetics2004 Jul 01; 167
AB  - The products of trithorax group (trxG) genes maintain active transcription of many important developmental regulatory genes, including homeotic genes. Several trxG proteins have been shown to act in multimeric protein complexes that modify chromatin structure. ASH2, the product of the Drosophila trxG gene absent, small, or homeotic discs 2 (ash2) is a component of a 500-kD complex. In this article, we provide biochemical evidence that ASH2 binds directly to Skittles (SKTL), a predicted phosphatidylinositol 4-phosphate 5-kinase, and genetic evidence that the association of these proteins is functionally significant. We also show that histone H1 hyperphosphorylation is dramatically increased in both ash2 and sktl mutant polytene chromosomes. These results suggest that ASH2 maintains active transcription by binding a producer of nuclear phosphoinositides and downregulating histone H1 hyperphosphorylation.