RT Journal Article
SR Electronic
T1 The BDGP Gene Disruption Project
JF Genetics
JO Genetics
FD Genetics Society of America
SP 761
OP 781
DO 10.1534/genetics.104.026427
VO 167
IS 2
A1 Bellen, Hugo J.
A1 Levis, Robert W.
A1 Liao, Guochun
A1 He, Yuchun
A1 Carlson, Joseph W.
A1 Tsang, Garson
A1 Evans-Holm, Martha
A1 Hiesinger, P. Robin
A1 Schulze, Karen L.
A1 Rubin, Gerald M.
A1 Hoskins, Roger A.
A1 Spradling, Allan C.
YR 2004
UL http://www.genetics.org/content/167/2/761.abstract
AB The Berkeley Drosophila Genome Project (BDGP) strives to disrupt each Drosophila gene by the insertion of a single transposable element. As part of this effort, transposons in >30,000 fly strains were localized and analyzed relative to predicted Drosophila gene structures. Approximately 6300 lines that maximize genomic coverage were selected to be sent to the Bloomington Stock Center for public distribution, bringing the size of the BDGP gene disruption collection to 7140 lines. It now includes individual lines predicted to disrupt 5362 of the 13,666 currently annotated Drosophila genes (39%). Other lines contain an insertion at least 2 kb from others in the collection and likely mutate additional incompletely annotated or uncharacterized genes and chromosomal regulatory elements. The remaining strains contain insertions likely to disrupt alternative gene promoters or to allow gene misexpression. The expanded BDGP gene disruption collection provides a public resource that will facilitate the application of Drosophila genetics to diverse biological problems. Finally, the project reveals new insight into how transposons interact with a eukaryotic genome and helps define optimal strategies for using insertional mutagenesis as a genomic tool.