RT Journal Article
SR Electronic
T1 Functional Dissection of the <em>cis</em>-Acting Sequences of the Arabidopsis Transposable Element <em>Tag1</em> Reveals Dissimilar Subterminal Sequence and Minimal Spacing Requirements for Transposition
JF Genetics
JO Genetics
FD Genetics Society of America
SP 817
OP 830
VO 157
IS 2
A1 Liu, Dong
A1 Mack, Alyson
A1 Wang, Rongchen
A1 Galli, Mary
A1 Belk, Jason
A1 Ketpura, Nan I.
A1 Crawford, Nigel M.
YR 2001
UL http://www.genetics.org/content/157/2/817.abstract
AB The Arabidopsis transposon Tag1 has an unusual subterminal structure containing four sets of dissimilar repeats: one set near the 5′ end and three near the 3′ end. To determine sequence requirements for efficient and regulated transposition, deletion derivatives of Tag1 were tested in Arabidopsis plants. These tests showed that a 98-bp 5′ fragment containing the 22-bp inverted repeat and four copies of the AAACCX (X = C, A, G) 5′ subterminal repeat is sufficient for transposition while a 52-bp 5′ fragment containing only one copy of the subterminal repeat is not. At the 3′ end, a 109-bp fragment containing four copies of the most 3′ repeat TGACCC, but not a 55-bp fragment, which has no copies of the subterminal repeats, is sufficient for transposition. The 5′ and 3′ end fragments are not functionally interchangeable and require an internal spacer DNA of minimal length between 238 and 325 bp to be active. Elements with these minimal requirements show transposition rates and developmental control of excision that are comparable to the autonomous Tag1 element. Last, a DNA-binding activity that interacts with the 3′ 109-bp fragment but not the 5′ 98-bp fragment of Tag1 was found in nuclear extracts of Arabidopsis plants devoid of Tag1.