PT  - JOURNAL ARTICLE
AU  - Suzuki, Go
AU  - Kai, Naoko
AU  - Hirose, Tamaki
AU  - Fukui, Kiichi
AU  - Nishio, Takeshi
AU  - Takayama, Seiji
AU  - Isogai, Akira
AU  - Watanabe, Masao
AU  - Hinata, Kokichi
TI  - Genomic Organization of the &lt;em&gt;S&lt;/em&gt; Locus: Identification and Characterization of Genes in &lt;em&gt;SLG&lt;/em&gt;/&lt;em&gt;SRK&lt;/em&gt; Region of &lt;em&gt;S&lt;sup&gt;9&lt;/sup&gt;&lt;/em&gt; Haplotype of &lt;em&gt;Brassica campestris&lt;/em&gt; (syn. &lt;em&gt;rapa&lt;/em&gt;)
DP  - 1999 Sep 01
TA  - Genetics
PG  - 391--400
VI  - 153
IP  - 1
4099  - http://www.genetics.org/content/153/1/391.short
4100  - http://www.genetics.org/content/153/1/391.full
SO  - Genetics1999 Sep 01; 153
AB  - In Brassica, two self-incompatibility genes, encoding SLG (S locus glycoprotein) and SRK (S-receptor kinase), are located at the S locus and expressed in the stigma. Recent molecular analysis has revealed that the S locus is highly polymorphic and contains several genes, i.e., SLG, SRK, the as-yet-unidentified pollen S gene(s), and other linked genes. In the present study, we searched for expressed sequences in a 76-kb SLG/SRK region of the S9 haplotype of Brassica campestris (syn. rapa) and identified 10 genes in addition to the four previously identified (SLG9, SRK9, SAE1, and SLL2) in this haplotype. This gene density (1 gene/5.4 kb) suggests that the S locus is embedded in a gene-rich region of the genome. The average G + C content in this region is 32.6%. An En/Spm-type transposon-like element was found downstream of SLG9. Among the genes we identified that had not previously been found to be linked to the S locus were genes encoding a small cysteine-rich protein, a J-domain protein, and an antisilencing protein (ASF1) homologue. The small cysteine-rich protein was similar to a pollen coat protein, named PCP-A1, which had previously been shown to bind SLG.