Files in this data supplement:
- Supporting Information: Figures S1-S2, Tables S1-S2, and Files S1-S2 (PDF, 1 MB)
- Figure S1: Spontaneous loss of SEC in intenstinal cells does not require a heat shock response. (PDF, 874 KB)
- Figure S2: Relationship between sgRNA score and recombination efficiency. (PDF, 545 KB)
- Table S1: Strains generated for this study. (PDF, 492 KB)
- Table S2: sgRNA targets used in this study. (PDF, 490 KB)
- File S1: Supplemental Text: Detailed protocol for generating Cas9-mediated fluorescent protein knock-ins with a self-excising selection cassette (SEC). (PDF, 678 KB)
- File S2: Movie S1: Plate phenotype of sqt-1(d) animals before and after marker excision. The left panel shows animals homozygous for a single-copy insertion of a sqt-1(d)::hygR selectable marker; L3 and older worms exhibit a strong and highly penetrant Roller phenotype. The right panel shows the same strain after marker excision; wild-type animals are easily recognizable. (.avi, 3 MB)