Supplement to Streamlined Genome Engineering with a Self-Excising Drug Selection Cassette | Genetics
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Research ArticleINVESTIGATION

Streamlined Genome Engineering with a Self-Excising Drug Selection Cassette

Daniel J. Dickinson, Ariel M. Pani, Jennifer K. Heppert, Christopher D. Higgins and Bob Goldstein
Genetics June 2015, genetics.115.178335; DOI: https://doi.org/10.1534/genetics.115.178335

Files in this data supplement:

  • Supporting Information: Figures S1-S2, Tables S1-S2, and Files S1-S2 (PDF, 1 MB)
  • Figure S1: Spontaneous loss of SEC in intenstinal cells does not require a heat shock response. (PDF, 874 KB)
  • Figure S2: Relationship between sgRNA score and recombination efficiency. (PDF, 545 KB)
  • Table S1: Strains generated for this study. (PDF, 492 KB)
  • Table S2: sgRNA targets used in this study. (PDF, 490 KB)
  • File S1: Supplemental Text: Detailed protocol for generating Cas9-mediated fluorescent protein knock-ins with a self-excising selection cassette (SEC). (PDF, 678 KB)
  • File S2: Movie S1: Plate phenotype of sqt-1(d) animals before and after marker excision. The left panel shows animals homozygous for a single-copy insertion of a sqt-1(d)::hygR selectable marker; L3 and older worms exhibit a strong and highly penetrant Roller phenotype. The right panel shows the same strain after marker excision; wild-type animals are easily recognizable. (.avi, 3 MB)

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