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Research ArticleINVESTIGATION

Multiplex Conditional Mutagenesis Using Transgenic Expression of Cas9 and sgRNAs

Linlin Yin, Lisette A. Maddison, Mingyu Li, Nergis Kara, Matthew C. LaFave, Gaurav K. Varshney, Shawn M. Burgess, James G. Patton and Wenbiao Chen
Genetics April 2015, genetics.115.176917; DOI: https://doi.org/10.1534/genetics.115.176917

Files in this data supplement:

  • Supporting Information: Figure S1, Tables S1-S5, and File S1 (PDF, 430 KB)
  • Figure S1: Alignment of top 5 alleles of the 3 loci in Tg(actb2:cas9; (U6x:sgRNA(insra/b) fish. (PDF, 707 KB)
  • Table S1: List of primers use in the study. (.xlsx, 11 KB)
  • Table S2: Mutation rates at the three loci calculated based on high throughput sequencing data. (.xlsx, 55 KB)
  • Table S3: Alleles and read counts of insra locus identified in the 17 double carriers. "D" denotes deletion. "I" denotes insertion. "C" denotes deletion and insertion. The first number indicates the position at which InDel occurs. The second number is the number of nucleotides of insertion or deletion in the allele. (.xlsx, 404 KB)
  • Table S4: Alleles and read counts of insrb locus identified in the 17 double carriers. (.xlsx, 264 KB)
  • Table S5: Alleles and read counts of tyr locus identified in the 17 double carriers. (.xlsx, 75 KB)
  • File S1: Supplemental Methods (PDF, 667 KB)

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