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Research ArticleNOTE

Genome engineering of Drosophila with the CRISPR RNA-guided Cas9 nuclease

Scott J. Gratz, Alexander M. Cummings, Jennifer N. Nguyen, Danielle C. Hamm, Laura K. Donohue, Melissa M. Harrison, Jill Wildonger and Kate M. O'Connor-Giles
Genetics May 2013, genetics.113.152710; DOI: https://doi.org/10.1534/genetics.113.152710
Scott J. Gratz
University of Wisconsin-Madison;
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Alexander M. Cummings
University of Wisconsin-Madison;
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Jennifer N. Nguyen
University of Wisconsin-Madison;
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Danielle C. Hamm
University of Wisconsin-Madison;
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Laura K. Donohue
University of Wisconsin-Madison;
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Melissa M. Harrison
University of Wisconsin School of Medicine and Public Health
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Jill Wildonger
University of Wisconsin-Madison;
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Kate M. O'Connor-Giles
University of Wisconsin-Madison;
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  • For correspondence: oconnorgiles@wisc.edu

Files in this data supplement:

  • Supporting Information: File S1, Figures S1-S3, Table S1, and Supporting References (PDF, 2 MB)
  • File S1: Materials and Methods (PDF, 71 KB)
  • Figure S1: CRISPR RNA/Cas9 molecular reagents used in this study (PDF, 844 KB)
  • Figure S2: Sequences of CRISPR RNA/Cas9 constructs (PDF, 94 KB)
  • Figure S3: Off-target analysis of germline transformants (PDF, 1 MB)
  • Table S1: chiRNA sequences used to target yellow and rosy (PDF, 71 KB)
  • Supporting References: PDF, 49 KB

  View article

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