Research ArticleREGULAR RESEARCH PAPERS

TORC2 Signaling is Antagonized by Protein Phosphatase 2A and the Far Complex in Saccharomyces cerevisiae

Tammy Pracheil, Janet Thornton and Zhengchang Liu
Genetics January 2012, genetics.111.138305; DOI: https://doi.org/10.1534/genetics.111.138305

Supporting Information for Pracheil, Thornton, and Liu, 2012

Supporting Information

  • Supporting Information - Figures S1-S8 and Tables S1-S3 (PDF, 605 KB)
  • Figure S1 - sac7Δ and far11Δ do not suppress the temperature-sensitive growth phenotype of a tor1Δ tor2-21 double mutant (PDF, 140 KB)
  • Figure S2 - The effect of rapamycin on the growth of sac7Δ and far11Δ mutant cells (PDF, 216 KB)
  • Figure S3 - A FAR11-HA fusion construct is functional (PDF, 114 KB)
  • Figure S4 - Far11-HA in total cellular proteins prepared by trichloroacetic acid precipitation exists as a single band on Western blots (PDF, 88 KB)
  • Figure S5 - The effect of far9Δ and far10Δ on suppressing the temperature-sensitive growth phenotype of a tor2-21 mutant (PDF, 93 KB)
  • Figure S6 - Tetrad analysis of sporulated diploid cells heterozygous for mutations in FAR11 and TOR2, AVO1 or AVO3 (PDF, 140 KB)
  • Figure S7 - (A) Tpd3-myc is functional (B) Pph21-myc is functional (PDF, 106 KB)
  • Figure S8 - Immunoblot analysis of HA-tagged Ypk2 (panel A) and Slm2 (panel B) (PDF, 104 KB)
  • Table S1 - Quantitative analysis of polarization of the actin cytoskeleton in wild-type and isogenic tor2-21 (SH121), tor2-21 sac7Δ (TPY110), tor2-21 far11Δ (TPY311), tor2-21 sac7Δ far11Δ (TPY301) mutant cells (PDF, 102 KB)
  • Table S2 - Strains used (PDF, 103 KB)
  • Table S3 - Plasmids used (PDF, 102 KB)

  View article