Jamie has a longstanding interest in understanding the Hedgehog signaling pathway. Previously, she investigated how Hedgehog signaling regulates Cubitus Interruptus (Ci), the singular transcription factor of the pathway. Using CRISPR, she developed Ci variants (crCi) to dissect the mechanism of Ci processing, activation, and stabilization. She found that in the absence of processing there is still normal graded activity and proteolysis of Ci. Moreover, the upstream regulators Costal2 and PKA are not only important for processing but also silence Ci-155 activity itself. More recently she has mapped the effects of the negative regulator Su(fu) and showed that Su(fu) inhibits Ci through N-terminus and C-terminus. Interestingly, there are certain regions on Ci that support Su(fu) inhibition of activity but have different effects on Ci stability.
