A PUF Hub drives self-renewal in Caenorhabditis elegans germline stem cells, pp. 147-161
Kimberly A. Haupt, Kimberley T. Law, Amy L. Enright, Charlotte R. Kanzler, Heaji Shin, Marvin Wickens, and Judith Kimble
The Caenorhabditis elegans network for germline stem cells (GSCs) was missing key intrinsic regulators of self-renewal. Haupt et al. report that these missing regulators are PUF RNA-binding proteins, PUF-3 and PUF-11, which together with FBF-1 and FBF-2, account for the effects of niche signaling on self-renewal. This discovery underscores the principle role of PUF RNA-binding proteins in stem cell maintenance and places them as central to a regulatory “PUF hub” that drives GSC self-renewal. This PUF hub, composed of four PUF proteins and two PUF partners, constitutes the intrinsic self-renewal node of the C. elegans GSC regulatory network.
The driver of extreme human-specific Olduvai repeat expansion remains highly active in the human genome, pp. 179-191
Ilea E. Heft, Yulia Mostovoy, Michal Levy-Sakin, Walfred Ma, Aaron J. Stevens, Steven Pastor, Jennifer McCaffrey, Dario Boffelli, David I. Martin, Ming Xiao, Martin A. Kennedy, Pui-Yan Kwok, and James M. Sikela
Olduvai (formerly DUF1220) protein domains have undergone the largest human-specific increase in copy number of any coding region in the genome (∼300 copies of which 165 are human-specific) and have been implicated in human brain evolution. By analyzing the human genome reference sequence (hg38) and single molecule optical mapping data of 186 individuals, Heft et al. show that the genomic mechanism that allowed this dramatic evolutionary copy number increase is also responsible for the extreme Olduvai variability found among present day human populations. In other words, their findings indicate the same process that may have been a key contributor to the expansion of the human brain remains highly active in present human populations.
An activating mutation in ERK causes hyperplastic tumors in a scribble mutant tissue in Drosophila, pp. 109-120
Tatyana Kushnir, Shaked Bar-Cohen, Navit Mooshayef, Rotem Lange, Allan Bar-Sinai, Helit Rozen, Adi Salzberg, David Engelberg, and Ze’ev Paroush
Excessive RTK signaling, often caused by activating mutations in Ras, Raf and/or MEK, occurs in most human tumors. Intriguingly, confirmed cancer-driver mutations in the downstream effector kinase, ERK, have not been reported. To test if active ERK mutants can function as oncoproteins, Kushnir et al. introduced an activating mutation, originally identified in a yeast ERK, into the single Drosophila ERK. The authors find that this mutation renders ERK catalytically active independently of upstream signaling, and that its expression induces extensive over-proliferation and hyperplastic tumor formation in vivo. Thus, some human ERK1/2 mutations identified in patient-derived tumors may actually represent overlooked oncogenic, cancer-causing mutations.
Rapid and predictable evolution of admixed populations between two Drosophila species pairs, pp. 211-230
Daniel R. Matute, Aaron A. Comeault, Eric Earley, Antonio Serrato-Capuchina, David Peede, Anaïs Monroy-Eklund, Wen Huang, Corbin D. Jones, Trudy F. C. Mackay, and Jerry A. Coyne
In this article, Matute et al. report an experiment in which they generated eight interspecific admixed populations using two species pairs of Drosophila. They found that in both species pairs, and across all experimental replicates, all phenotypes (morphology, behavior, and fertility) rapidly regressed to those of the parental continental species, becoming indistinguishable from that species. Consistent with this observation, the genomes of the admixed populations also regressed to the continental species with only some traces of the island species. Their results show that the evolutionary outcome of hybridization can be highly repeatable and predictable at least in hybridizing species of Drosophila.
In CBE — Life Sciences Education (LSE)
Expert-novice comparison reveals pedagogical implications for students' analysis of primary literature, CBE—Life Sci. Edu. 18:4, DOI:10.1187/cbe.18-05-0077
April A. Nelms and Miriam Segura-Totten
There are many reasons to engage undergraduates in reading primary literature, including increasing students’ scientific literacy, ability to analyze data, and critical thinking skills. However, students often struggle with this process, prompting faculty to develop mechanisms to facilitate student involvement with the text. To better understand the differences in how experts and novices approach reading primary literature, Nelms and Segura-Totten analyzed think-aloud interviews of both faculty and students as they read a scientific article. The authors utilized both cognitive load theory and the interactive, constructive, active, and passive (ICAP) framework to interpret the results, identifying approaches and mechanisms that experts use to a greater extent than novices. Nelms and Segura-Totten use these findings to offer specific recommendations to faculty seeking help teach students to read primary literature.
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