Improved Activities of CREB Binding Protein, Heterogeneous Nuclear Ribonucleoproteins and Proteasome Following Downregulation of Noncoding hsrω Transcripts Help Suppress Poly(Q) Pathogenesis in Fly Models

doi:10.1534/genetics.119.302466

In the article by M. Mallik and S. C. Lakhotia (GENETICS 184: 927–945) entitled “Improved Activities of CREB Binding Protein, Heterogeneous Nuclear Ribonucleoproteins and Proteasome Following Downregulation of Noncoding hsrω Transcripts Help Suppress Poly(Q) Pathogenesis in Fly Models,” a duplicate image was erroneously included in Figure 9B on page 940. Figure 9B has now been corrected as below.

Figure 9.

Functional proteasomal activity is required for suppression of poly(Q) damage to eyes and for suppression of formation of IBs in eye discs. A–D and I–L are photomicrographs and E–H and M–P are nail polish imprints of the eye surface. Genotypes common to rows are indicated on the left while those for columns are indicated at the top. (A′–D′) Confocal projections of third instar larval eye imaginal discs immunoassayed for the poly(Q) protein (red, A′–D′). (Q–T) Confocal projections showing Ub^G76V-GFP reporter activity in eye imaginal discs from GMR ‐GAL4 UAS-127Q/+; +/+ (Q), GMR-GAL4 UAS-127Q/+; hsrω-RNAi/+ (R), GMR-GAL4 UAS-MJDtrQ78(S)/+; +/+ (S), and GMR-GAL4 UAS-MJDtrQ78(S)/+; hsrω-RNAi³/+ (T) third instar larvae. Bars for A–D and I–L in D, for E–H and M–P in H, and for A′–D′ and Q–T in D′: 20 μm. Inset in R shows semiquantitative RT–PCR levels of EGFP, hsrω-n, and 127Q transcripts in total RNA from heads of w/w; GMR-GAL4 UAS-127Q/+; +/+ (lane 1) and w/w; GMR-GAL4 UAS-127Q/+; hsrω-RNAi³/+ (lane 2) adult flies, respectively. G3PDH was coamplified and used as a loading control.