The Transgenic RNAi Project at Harvard Medical School: Resources and Validation
Lizabeth A. Perkins, Laura Holderbaum, Rong Tao, Yanhui Hu, Richelle Sopko, Kim McCall, Donghui Yang-Zhou, Ian Flockhart, Richard Binari, Hye-Seok Shim, Audrey Miller, Amy Housden, Marianna Foos, Sakara Randkelv, Colleen Kelley, Pema Namgyal, Christians Villalta, Lu-Ping Liu, Xia Jiang, Qiao Huan-Huan, Xia Wang, Asao Fujiyama, Atsushi Toyoda, Kathleen Ayers, Allison Blum, Benjamin Czech, Ralph Neumuller, Dong Yan, Amanda Cavallaro, Karen Hibbard, Don Hall, Lynn Cooley, Gregory J. Hannon, Ruth Lehmann, Annette Parks, Stephanie E. Mohr, Ryu Ueda, Shu Kondo, Jian-Quan Ni and Norbert Perrimon
Genetics November 1, 2015 vol. 201 no. 3 843-852; https://doi.org/10.1534/genetics.115.180208
Lizabeth A. Perkins
*Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Laura Holderbaum
*Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Rong Tao
*Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Yanhui Hu
*Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Richelle Sopko
*Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Kim McCall
†Boston University, Boston, Massachusetts 02215
Donghui Yang-Zhou
*Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Ian Flockhart
*Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Richard Binari
*Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
‡Howard Hughes Medical Institute, Boston, Massachusetts 02115
Hye-Seok Shim
*Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Audrey Miller
*Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Amy Housden
*Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Marianna Foos
*Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Sakara Randkelv
*Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Colleen Kelley
*Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Pema Namgyal
*Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Christians Villalta
*Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
‡Howard Hughes Medical Institute, Boston, Massachusetts 02115
Lu-Ping Liu
*Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
§TsingHua Fly Center, Beijing, 100084, China
Xia Jiang
§TsingHua Fly Center, Beijing, 100084, China
Qiao Huan-Huan
§TsingHua Fly Center, Beijing, 100084, China
Xia Wang
§TsingHua Fly Center, Beijing, 100084, China
Asao Fujiyama
**Comparative Genomics Laboratory, National Institute of Genetics, Shizuoka 411-8540, Japan
Atsushi Toyoda
**Comparative Genomics Laboratory, National Institute of Genetics, Shizuoka 411-8540, Japan
Kathleen Ayers
††Department of Genetics, Yale University, New Haven, Connecticut 06510
Allison Blum
‡Howard Hughes Medical Institute, Boston, Massachusetts 02115
‡‡Skirball Institute, Department of Cell Biology, New York University School of Medicine, New York, New York 10016
Benjamin Czech
§§CRUK Cambridge Institute, University of Cambridge, Cambridge, CB2 1TN, United Kingdom
Ralph Neumuller
*Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Dong Yan
*Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Amanda Cavallaro
‡Howard Hughes Medical Institute, Boston, Massachusetts 02115
***Janelia Farm Research Institute ,Asburn, Virginia, 20147
Karen Hibbard
‡Howard Hughes Medical Institute, Boston, Massachusetts 02115
***Janelia Farm Research Institute ,Asburn, Virginia, 20147
Don Hall
‡Howard Hughes Medical Institute, Boston, Massachusetts 02115
***Janelia Farm Research Institute ,Asburn, Virginia, 20147
Lynn Cooley
††Department of Genetics, Yale University, New Haven, Connecticut 06510
Gregory J. Hannon
§§CRUK Cambridge Institute, University of Cambridge, Cambridge, CB2 1TN, United Kingdom
Ruth Lehmann
‡Howard Hughes Medical Institute, Boston, Massachusetts 02115
‡‡Skirball Institute, Department of Cell Biology, New York University School of Medicine, New York, New York 10016
Annette Parks
†††Bloomington Stock Center Bloomington, Indiana, 47405
Stephanie E. Mohr
*Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Ryu Ueda
**Comparative Genomics Laboratory, National Institute of Genetics, Shizuoka 411-8540, Japan
Shu Kondo
*Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
‡‡‡Invertebrate Genetics Laboratory, National Institute of Genetics, Shizuoka 411-8540, Japan
Jian-Quan Ni
*Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
§TsingHua Fly Center, Beijing, 100084, China
Norbert Perrimon
*Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
‡Howard Hughes Medical Institute, Boston, Massachusetts 02115
Data supplements
Files in this data supplement:
- Supporting Information: Tables S1-S3 and Figure S1 (PDF, 932 KB)
- Table S1: The TRiP Toolbox Stocks. (PDF, 43 KB)
- Table S2: D. persimilis rescue fosmids. (PDF, 85 KB)
- Table S3: C911 short hairpin designs. (PDF, 63 KB)
- Figure S1: The TRiP Valium series vectors. (PDF, 856 KB)
PUBLICATION INFORMATION
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The Transgenic RNAi Project at Harvard Medical School: Resources and Validation
Lizabeth A. Perkins, Laura Holderbaum, Rong Tao, Yanhui Hu, Richelle Sopko, Kim McCall, Donghui Yang-Zhou, Ian Flockhart, Richard Binari, Hye-Seok Shim, Audrey Miller, Amy Housden, Marianna Foos, Sakara Randkelv, Colleen Kelley, Pema Namgyal, Christians Villalta, Lu-Ping Liu, Xia Jiang, Qiao Huan-Huan, Xia Wang, Asao Fujiyama, Atsushi Toyoda, Kathleen Ayers, Allison Blum, Benjamin Czech, Ralph Neumuller, Dong Yan, Amanda Cavallaro, Karen Hibbard, Don Hall, Lynn Cooley, Gregory J. Hannon, Ruth Lehmann, Annette Parks, Stephanie E. Mohr, Ryu Ueda, Shu Kondo, Jian-Quan Ni and Norbert Perrimon
Genetics November 1, 2015 vol. 201 no. 3 843-852; https://doi.org/10.1534/genetics.115.180208
Lizabeth A. Perkins
*Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Laura Holderbaum
*Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Rong Tao
*Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Yanhui Hu
*Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Richelle Sopko
*Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Kim McCall
†Boston University, Boston, Massachusetts 02215
Donghui Yang-Zhou
*Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Ian Flockhart
*Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Richard Binari
*Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
‡Howard Hughes Medical Institute, Boston, Massachusetts 02115
Hye-Seok Shim
*Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Audrey Miller
*Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Amy Housden
*Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Marianna Foos
*Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Sakara Randkelv
*Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Colleen Kelley
*Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Pema Namgyal
*Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Christians Villalta
*Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
‡Howard Hughes Medical Institute, Boston, Massachusetts 02115
Lu-Ping Liu
*Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
§TsingHua Fly Center, Beijing, 100084, China
Xia Jiang
§TsingHua Fly Center, Beijing, 100084, China
Qiao Huan-Huan
§TsingHua Fly Center, Beijing, 100084, China
Xia Wang
§TsingHua Fly Center, Beijing, 100084, China
Asao Fujiyama
**Comparative Genomics Laboratory, National Institute of Genetics, Shizuoka 411-8540, Japan
Atsushi Toyoda
**Comparative Genomics Laboratory, National Institute of Genetics, Shizuoka 411-8540, Japan
Kathleen Ayers
††Department of Genetics, Yale University, New Haven, Connecticut 06510
Allison Blum
‡Howard Hughes Medical Institute, Boston, Massachusetts 02115
‡‡Skirball Institute, Department of Cell Biology, New York University School of Medicine, New York, New York 10016
Benjamin Czech
§§CRUK Cambridge Institute, University of Cambridge, Cambridge, CB2 1TN, United Kingdom
Ralph Neumuller
*Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Dong Yan
*Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Amanda Cavallaro
‡Howard Hughes Medical Institute, Boston, Massachusetts 02115
***Janelia Farm Research Institute ,Asburn, Virginia, 20147
Karen Hibbard
‡Howard Hughes Medical Institute, Boston, Massachusetts 02115
***Janelia Farm Research Institute ,Asburn, Virginia, 20147
Don Hall
‡Howard Hughes Medical Institute, Boston, Massachusetts 02115
***Janelia Farm Research Institute ,Asburn, Virginia, 20147
Lynn Cooley
††Department of Genetics, Yale University, New Haven, Connecticut 06510
Gregory J. Hannon
§§CRUK Cambridge Institute, University of Cambridge, Cambridge, CB2 1TN, United Kingdom
Ruth Lehmann
‡Howard Hughes Medical Institute, Boston, Massachusetts 02115
‡‡Skirball Institute, Department of Cell Biology, New York University School of Medicine, New York, New York 10016
Annette Parks
†††Bloomington Stock Center Bloomington, Indiana, 47405
Stephanie E. Mohr
*Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Ryu Ueda
**Comparative Genomics Laboratory, National Institute of Genetics, Shizuoka 411-8540, Japan
Shu Kondo
*Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
‡‡‡Invertebrate Genetics Laboratory, National Institute of Genetics, Shizuoka 411-8540, Japan
Jian-Quan Ni
*Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
§TsingHua Fly Center, Beijing, 100084, China
Norbert Perrimon
*Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
‡Howard Hughes Medical Institute, Boston, Massachusetts 02115
The Transgenic RNAi Project at Harvard Medical School: Resources and Validation
Lizabeth A. Perkins, Laura Holderbaum, Rong Tao, Yanhui Hu, Richelle Sopko, Kim McCall, Donghui Yang-Zhou, Ian Flockhart, Richard Binari, Hye-Seok Shim, Audrey Miller, Amy Housden, Marianna Foos, Sakara Randkelv, Colleen Kelley, Pema Namgyal, Christians Villalta, Lu-Ping Liu, Xia Jiang, Qiao Huan-Huan, Xia Wang, Asao Fujiyama, Atsushi Toyoda, Kathleen Ayers, Allison Blum, Benjamin Czech, Ralph Neumuller, Dong Yan, Amanda Cavallaro, Karen Hibbard, Don Hall, Lynn Cooley, Gregory J. Hannon, Ruth Lehmann, Annette Parks, Stephanie E. Mohr, Ryu Ueda, Shu Kondo, Jian-Quan Ni and Norbert Perrimon
Genetics November 1, 2015 vol. 201 no. 3 843-852; https://doi.org/10.1534/genetics.115.180208
Lizabeth A. Perkins
*Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Laura Holderbaum
*Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Rong Tao
*Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Yanhui Hu
*Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Richelle Sopko
*Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Kim McCall
†Boston University, Boston, Massachusetts 02215
Donghui Yang-Zhou
*Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Ian Flockhart
*Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Richard Binari
*Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
‡Howard Hughes Medical Institute, Boston, Massachusetts 02115
Hye-Seok Shim
*Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Audrey Miller
*Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Amy Housden
*Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Marianna Foos
*Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Sakara Randkelv
*Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Colleen Kelley
*Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Pema Namgyal
*Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Christians Villalta
*Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
‡Howard Hughes Medical Institute, Boston, Massachusetts 02115
Lu-Ping Liu
*Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
§TsingHua Fly Center, Beijing, 100084, China
Xia Jiang
§TsingHua Fly Center, Beijing, 100084, China
Qiao Huan-Huan
§TsingHua Fly Center, Beijing, 100084, China
Xia Wang
§TsingHua Fly Center, Beijing, 100084, China
Asao Fujiyama
**Comparative Genomics Laboratory, National Institute of Genetics, Shizuoka 411-8540, Japan
Atsushi Toyoda
**Comparative Genomics Laboratory, National Institute of Genetics, Shizuoka 411-8540, Japan
Kathleen Ayers
††Department of Genetics, Yale University, New Haven, Connecticut 06510
Allison Blum
‡Howard Hughes Medical Institute, Boston, Massachusetts 02115
‡‡Skirball Institute, Department of Cell Biology, New York University School of Medicine, New York, New York 10016
Benjamin Czech
§§CRUK Cambridge Institute, University of Cambridge, Cambridge, CB2 1TN, United Kingdom
Ralph Neumuller
*Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Dong Yan
*Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Amanda Cavallaro
‡Howard Hughes Medical Institute, Boston, Massachusetts 02115
***Janelia Farm Research Institute ,Asburn, Virginia, 20147
Karen Hibbard
‡Howard Hughes Medical Institute, Boston, Massachusetts 02115
***Janelia Farm Research Institute ,Asburn, Virginia, 20147
Don Hall
‡Howard Hughes Medical Institute, Boston, Massachusetts 02115
***Janelia Farm Research Institute ,Asburn, Virginia, 20147
Lynn Cooley
††Department of Genetics, Yale University, New Haven, Connecticut 06510
Gregory J. Hannon
§§CRUK Cambridge Institute, University of Cambridge, Cambridge, CB2 1TN, United Kingdom
Ruth Lehmann
‡Howard Hughes Medical Institute, Boston, Massachusetts 02115
‡‡Skirball Institute, Department of Cell Biology, New York University School of Medicine, New York, New York 10016
Annette Parks
†††Bloomington Stock Center Bloomington, Indiana, 47405
Stephanie E. Mohr
*Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Ryu Ueda
**Comparative Genomics Laboratory, National Institute of Genetics, Shizuoka 411-8540, Japan
Shu Kondo
*Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
‡‡‡Invertebrate Genetics Laboratory, National Institute of Genetics, Shizuoka 411-8540, Japan
Jian-Quan Ni
*Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
§TsingHua Fly Center, Beijing, 100084, China
Norbert Perrimon
*Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
‡Howard Hughes Medical Institute, Boston, Massachusetts 02115