The Transgenic RNAi Project at Harvard Medical School: Resources and Validation
Lizabeth A. Perkins, Laura Holderbaum, Rong Tao, Yanhui Hu, Richelle Sopko, Kim McCall, Donghui Yang-Zhou, Ian Flockhart, Richard Binari, Hye-Seok Shim, Audrey Miller, Amy Housden, Marianna Foos, Sakara Randkelv, Colleen Kelley, Pema Namgyal, Christians Villalta, Lu-Ping Liu, Xia Jiang, Qiao Huan-Huan, Xia Wang, Asao Fujiyama, Atsushi Toyoda, Kathleen Ayers, Allison Blum, Benjamin Czech, Ralph Neumuller, Dong Yan, Amanda Cavallaro, Karen Hibbard, Don Hall, Lynn Cooley, Gregory J. Hannon, Ruth Lehmann, Annette Parks, Stephanie E. Mohr, Ryu Ueda, Shu Kondo, Jian-Quan Ni and Norbert Perrimon
Genetics November 1, 2015 vol. 201 no. 3 843-852; https://doi.org/10.1534/genetics.115.180208
Lizabeth A. Perkins
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Laura Holderbaum
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Rong Tao
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Yanhui Hu
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Richelle Sopko
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Kim McCall
Boston University, Boston, Massachusetts 02215
Donghui Yang-Zhou
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Ian Flockhart
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Richard Binari
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115Howard Hughes Medical Institute, Boston, Massachusetts 02115
Hye-Seok Shim
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Audrey Miller
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Amy Housden
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Marianna Foos
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Sakara Randkelv
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Colleen Kelley
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Pema Namgyal
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Christians Villalta
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115Howard Hughes Medical Institute, Boston, Massachusetts 02115
Lu-Ping Liu
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115TsingHua Fly Center, Beijing, 100084, China
Xia Jiang
TsingHua Fly Center, Beijing, 100084, China
Qiao Huan-Huan
TsingHua Fly Center, Beijing, 100084, China
Xia Wang
TsingHua Fly Center, Beijing, 100084, China
Asao Fujiyama
Comparative Genomics Laboratory, National Institute of Genetics, Shizuoka 411-8540, Japan
Atsushi Toyoda
Comparative Genomics Laboratory, National Institute of Genetics, Shizuoka 411-8540, Japan
Kathleen Ayers
Department of Genetics, Yale University, New Haven, Connecticut 06510
Allison Blum
Howard Hughes Medical Institute, Boston, Massachusetts 02115Skirball Institute, Department of Cell Biology, New York University School of Medicine, New York, New York 10016
Benjamin Czech
CRUK Cambridge Institute, University of Cambridge, Cambridge, CB2 1TN, United Kingdom
Ralph Neumuller
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Dong Yan
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Amanda Cavallaro
Howard Hughes Medical Institute, Boston, Massachusetts 02115Janelia Farm Research Institute ,Asburn, Virginia, 20147
Karen Hibbard
Howard Hughes Medical Institute, Boston, Massachusetts 02115Janelia Farm Research Institute ,Asburn, Virginia, 20147
Don Hall
Howard Hughes Medical Institute, Boston, Massachusetts 02115Janelia Farm Research Institute ,Asburn, Virginia, 20147
Lynn Cooley
Department of Genetics, Yale University, New Haven, Connecticut 06510
Gregory J. Hannon
CRUK Cambridge Institute, University of Cambridge, Cambridge, CB2 1TN, United Kingdom
Ruth Lehmann
Howard Hughes Medical Institute, Boston, Massachusetts 02115Skirball Institute, Department of Cell Biology, New York University School of Medicine, New York, New York 10016
Annette Parks
Bloomington Drosophila Stock Center Bloomington, Indiana, 47405
Stephanie E. Mohr
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Ryu Ueda
Comparative Genomics Laboratory, National Institute of Genetics, Shizuoka 411-8540, Japan
Shu Kondo
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115Invertebrate Genetics Laboratory, National Institute of Genetics, Shizuoka 411-8540, Japan
Jian-Quan Ni
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115TsingHua Fly Center, Beijing, 100084, China
Norbert Perrimon
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115Howard Hughes Medical Institute, Boston, Massachusetts 02115
Data supplements
Files in this data supplement:
- Supporting Information: Tables S1-S3 and Figure S1 (PDF, 932 KB)
- Table S1: The TRiP Toolbox Stocks. (PDF, 43 KB)
- Table S2: D. persimilis rescue fosmids. (PDF, 85 KB)
- Table S3: C911 short hairpin designs. (PDF, 63 KB)
- Figure S1: The TRiP Valium series vectors. (PDF, 856 KB)
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The Transgenic RNAi Project at Harvard Medical School: Resources and Validation
Lizabeth A. Perkins, Laura Holderbaum, Rong Tao, Yanhui Hu, Richelle Sopko, Kim McCall, Donghui Yang-Zhou, Ian Flockhart, Richard Binari, Hye-Seok Shim, Audrey Miller, Amy Housden, Marianna Foos, Sakara Randkelv, Colleen Kelley, Pema Namgyal, Christians Villalta, Lu-Ping Liu, Xia Jiang, Qiao Huan-Huan, Xia Wang, Asao Fujiyama, Atsushi Toyoda, Kathleen Ayers, Allison Blum, Benjamin Czech, Ralph Neumuller, Dong Yan, Amanda Cavallaro, Karen Hibbard, Don Hall, Lynn Cooley, Gregory J. Hannon, Ruth Lehmann, Annette Parks, Stephanie E. Mohr, Ryu Ueda, Shu Kondo, Jian-Quan Ni and Norbert Perrimon
Genetics November 1, 2015 vol. 201 no. 3 843-852; https://doi.org/10.1534/genetics.115.180208
Lizabeth A. Perkins
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Laura Holderbaum
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Rong Tao
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Yanhui Hu
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Richelle Sopko
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Kim McCall
Boston University, Boston, Massachusetts 02215
Donghui Yang-Zhou
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Ian Flockhart
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Richard Binari
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115Howard Hughes Medical Institute, Boston, Massachusetts 02115
Hye-Seok Shim
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Audrey Miller
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Amy Housden
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Marianna Foos
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Sakara Randkelv
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Colleen Kelley
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Pema Namgyal
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Christians Villalta
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115Howard Hughes Medical Institute, Boston, Massachusetts 02115
Lu-Ping Liu
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115TsingHua Fly Center, Beijing, 100084, China
Xia Jiang
TsingHua Fly Center, Beijing, 100084, China
Qiao Huan-Huan
TsingHua Fly Center, Beijing, 100084, China
Xia Wang
TsingHua Fly Center, Beijing, 100084, China
Asao Fujiyama
Comparative Genomics Laboratory, National Institute of Genetics, Shizuoka 411-8540, Japan
Atsushi Toyoda
Comparative Genomics Laboratory, National Institute of Genetics, Shizuoka 411-8540, Japan
Kathleen Ayers
Department of Genetics, Yale University, New Haven, Connecticut 06510
Allison Blum
Howard Hughes Medical Institute, Boston, Massachusetts 02115Skirball Institute, Department of Cell Biology, New York University School of Medicine, New York, New York 10016
Benjamin Czech
CRUK Cambridge Institute, University of Cambridge, Cambridge, CB2 1TN, United Kingdom
Ralph Neumuller
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Dong Yan
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Amanda Cavallaro
Howard Hughes Medical Institute, Boston, Massachusetts 02115Janelia Farm Research Institute ,Asburn, Virginia, 20147
Karen Hibbard
Howard Hughes Medical Institute, Boston, Massachusetts 02115Janelia Farm Research Institute ,Asburn, Virginia, 20147
Don Hall
Howard Hughes Medical Institute, Boston, Massachusetts 02115Janelia Farm Research Institute ,Asburn, Virginia, 20147
Lynn Cooley
Department of Genetics, Yale University, New Haven, Connecticut 06510
Gregory J. Hannon
CRUK Cambridge Institute, University of Cambridge, Cambridge, CB2 1TN, United Kingdom
Ruth Lehmann
Howard Hughes Medical Institute, Boston, Massachusetts 02115Skirball Institute, Department of Cell Biology, New York University School of Medicine, New York, New York 10016
Annette Parks
Bloomington Drosophila Stock Center Bloomington, Indiana, 47405
Stephanie E. Mohr
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Ryu Ueda
Comparative Genomics Laboratory, National Institute of Genetics, Shizuoka 411-8540, Japan
Shu Kondo
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115Invertebrate Genetics Laboratory, National Institute of Genetics, Shizuoka 411-8540, Japan
Jian-Quan Ni
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115TsingHua Fly Center, Beijing, 100084, China
Norbert Perrimon
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115Howard Hughes Medical Institute, Boston, Massachusetts 02115
The Transgenic RNAi Project at Harvard Medical School: Resources and Validation
Lizabeth A. Perkins, Laura Holderbaum, Rong Tao, Yanhui Hu, Richelle Sopko, Kim McCall, Donghui Yang-Zhou, Ian Flockhart, Richard Binari, Hye-Seok Shim, Audrey Miller, Amy Housden, Marianna Foos, Sakara Randkelv, Colleen Kelley, Pema Namgyal, Christians Villalta, Lu-Ping Liu, Xia Jiang, Qiao Huan-Huan, Xia Wang, Asao Fujiyama, Atsushi Toyoda, Kathleen Ayers, Allison Blum, Benjamin Czech, Ralph Neumuller, Dong Yan, Amanda Cavallaro, Karen Hibbard, Don Hall, Lynn Cooley, Gregory J. Hannon, Ruth Lehmann, Annette Parks, Stephanie E. Mohr, Ryu Ueda, Shu Kondo, Jian-Quan Ni and Norbert Perrimon
Genetics November 1, 2015 vol. 201 no. 3 843-852; https://doi.org/10.1534/genetics.115.180208
Lizabeth A. Perkins
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Laura Holderbaum
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Rong Tao
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Yanhui Hu
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Richelle Sopko
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Kim McCall
Boston University, Boston, Massachusetts 02215
Donghui Yang-Zhou
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Ian Flockhart
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Richard Binari
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115Howard Hughes Medical Institute, Boston, Massachusetts 02115
Hye-Seok Shim
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Audrey Miller
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Amy Housden
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Marianna Foos
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Sakara Randkelv
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Colleen Kelley
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Pema Namgyal
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Christians Villalta
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115Howard Hughes Medical Institute, Boston, Massachusetts 02115
Lu-Ping Liu
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115TsingHua Fly Center, Beijing, 100084, China
Xia Jiang
TsingHua Fly Center, Beijing, 100084, China
Qiao Huan-Huan
TsingHua Fly Center, Beijing, 100084, China
Xia Wang
TsingHua Fly Center, Beijing, 100084, China
Asao Fujiyama
Comparative Genomics Laboratory, National Institute of Genetics, Shizuoka 411-8540, Japan
Atsushi Toyoda
Comparative Genomics Laboratory, National Institute of Genetics, Shizuoka 411-8540, Japan
Kathleen Ayers
Department of Genetics, Yale University, New Haven, Connecticut 06510
Allison Blum
Howard Hughes Medical Institute, Boston, Massachusetts 02115Skirball Institute, Department of Cell Biology, New York University School of Medicine, New York, New York 10016
Benjamin Czech
CRUK Cambridge Institute, University of Cambridge, Cambridge, CB2 1TN, United Kingdom
Ralph Neumuller
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Dong Yan
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Amanda Cavallaro
Howard Hughes Medical Institute, Boston, Massachusetts 02115Janelia Farm Research Institute ,Asburn, Virginia, 20147
Karen Hibbard
Howard Hughes Medical Institute, Boston, Massachusetts 02115Janelia Farm Research Institute ,Asburn, Virginia, 20147
Don Hall
Howard Hughes Medical Institute, Boston, Massachusetts 02115Janelia Farm Research Institute ,Asburn, Virginia, 20147
Lynn Cooley
Department of Genetics, Yale University, New Haven, Connecticut 06510
Gregory J. Hannon
CRUK Cambridge Institute, University of Cambridge, Cambridge, CB2 1TN, United Kingdom
Ruth Lehmann
Howard Hughes Medical Institute, Boston, Massachusetts 02115Skirball Institute, Department of Cell Biology, New York University School of Medicine, New York, New York 10016
Annette Parks
Bloomington Drosophila Stock Center Bloomington, Indiana, 47405
Stephanie E. Mohr
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
Ryu Ueda
Comparative Genomics Laboratory, National Institute of Genetics, Shizuoka 411-8540, Japan
Shu Kondo
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115Invertebrate Genetics Laboratory, National Institute of Genetics, Shizuoka 411-8540, Japan
Jian-Quan Ni
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115TsingHua Fly Center, Beijing, 100084, China
Norbert Perrimon
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115Howard Hughes Medical Institute, Boston, Massachusetts 02115