Data supplements
Supporting Information
Supporting Information
- Supporting Information - Figures S1-S4 (PDF, 463 KB)
- Figure S1 - A. Yeast cells with indicated strain background were transformed with plasmid p413CUP1-NMGFP (NMGFP↑) or pRS413CUP1-NM (NM↑), and also one of the following plasmids: p426GPD-SWI1 (Swi1↑), p416GPD-URE2NPDGFP (Ure2↑), p426GPD-Q103GFP (Q103↑) or p426GPD-GFP (GFP↑).B. Randomly selected [PSI+] isolates shown in panel A were replica-plated onto GdnHCl-containing plates for up to three times (3x) and then patched back to YPD plates to see the curability and to determine [PSI+] variants. (PDF, 185 KB)
- Figure S2 - A. Sup35NMGFP and Swi1 were co-overproduced in a non-prion strain containing the plasmids p413CUP1-NMGFP and p426GPD-SWI1 (blue) in the presence of 100 µM CuSO4. B. Representative images of ring/rod Sup35NMGFP aggregates in pre-mature [PSI+] cells generated by co-overproduction of Sup35NMGFP and one of the indicated proteins. C. Sup35NMGFP aggregates of mature [PSI+] isolates obtained in experiments shown in Figure S1A-1B. (PDF, 148 KB)
- Figure S3 - A. Majority [PSI+] isolates obtained by co-production of Sup35NMGFP and Swi1 (Swi1 ↑), Ure21-65-GFP (Ure2 ↑) or polyQ103-GFP (Q103↑) remained [pin-]. B. Heritable Rnq1CFP aggregates (indicative of [PIN+]) were analyzed after eliminating the plasmid pRS413CUP1-NMGFP and p426GPD-SWI1. C. A [PSI+] isolate acquired by co-overproduction of Sup35NMYFP and Swi1 was compared with an isogenic [pin-][psi-] strain for their ability in promoting spontaneous [PIN+] conversion after incubation at 4ºC for the indicated days. D. The acquired Rnq1CFP aggregate-containing [PSI+] cells (20 days in panel C) were further stabilized by passages and compared with the initial [PSI+] strain (0 day cells) using a centrifugation assay. (PDF, 105 KB)
- Figure S4 - Representative images of Swi1-NQYFP and Sup35NM-CFP fluorescence patterns in pre-mature [PSI+] cells. (PDF, 200 KB)
