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A Gain-of-Function Screen Identifying Genes Required for Vein Formation in the Drosophila melanogaster Wing

Cristina Molnar, Ana López-Varea, Rosario Hernández and Jose F. de Celis
Genetics November 1, 2006 vol. 174 no. 3 1635-1659; https://doi.org/10.1534/genetics.106.061283
Cristina Molnar
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Ana López-Varea
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Rosario Hernández
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Jose F. de Celis
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Abstract

The formation of the Drosophila wing involves developmental processes such as cell proliferation, pattern formation, and cell differentiation that are common to all multicellular organisms. The genes controlling these cellular behaviors are conserved throughout the animal kingdom, and the genetic analysis of wing development has been instrumental in their identification and functional characterization. The wing is a postembryonic structure, and most loss-of-function mutations are lethal in homozygous flies before metamorphosis. In this manner, loss-of-function genetic screens aiming to identify genes affecting wing formation have not been systematically utilized. As an alternative, a number of genetic searches have utilized the phenotypic consequences of gene gain-of-expression, as a method more efficient to search for genes required during imaginal development. Here we present the results of a gain-of-function screen designed to identify genes involved in the formation of the wing veins. We generated 13,000 P-GS insertions of a P element containing UAS sequences (P-GS) and combined them with a Gal4 driver expressed mainly in the developing pupal veins. We selected 500 P-GSs that, in combination with the Gal4 driver, result in modifications of the veins, changes in the morphology of the wing, or defects in the differentiation of the trichomes. The P-element insertion sites were mapped to the genomic sequence, identifying 373 gene candidates to participate in wing morphogenesis and vein formation.

Footnotes

  • Communicating editor: K. G. Golic

  • Received May 24, 2006.
  • Accepted August 21, 2006.
  • Copyright © 2006 by the Genetics Society of America
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PUBLICATION INFORMATION

Volume 174 Issue 3, November 2006

Genetics: 174 (3)

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A Gain-of-Function Screen Identifying Genes Required for Vein Formation in the Drosophila melanogaster Wing

Cristina Molnar, Ana López-Varea, Rosario Hernández and Jose F. de Celis
Genetics November 1, 2006 vol. 174 no. 3 1635-1659; https://doi.org/10.1534/genetics.106.061283
Cristina Molnar
  • Find this author on Google Scholar
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Ana López-Varea
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Rosario Hernández
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Jose F. de Celis
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Citation

A Gain-of-Function Screen Identifying Genes Required for Vein Formation in the Drosophila melanogaster Wing

Cristina Molnar, Ana López-Varea, Rosario Hernández and Jose F. de Celis
Genetics November 1, 2006 vol. 174 no. 3 1635-1659; https://doi.org/10.1534/genetics.106.061283
Cristina Molnar
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ana López-Varea
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Rosario Hernández
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jose F. de Celis
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