Natural Wolbachia Infections in the Drosophila yakuba Species Complex Do Not Induce Cytoplasmic Incompatibility but Fully Rescue the wRi Modification

Article Figures & Data

TABLE 1

Drosophila species and strains used in this study and their associated Wolbachia strain

Species	Strain	Source	Wolbachia^a
D. yakuba	SA3	Bom Successo, Africa^b	wSty
D. yakuba	0261.0^c	NDSRC	—
D. yakuba	KB82	This study	wRi
D. yakuba	KB83	This study	wRi
D. teissieri	S-13a	Umea	wSty
D. teissieri	S-33	Cambridge	wSty
D. teissieri	0257.0^d	NDSRC	wSty
D. teissieri	1015	Bloomington	wSty
D. teissieri	KB114	This study	wRi
D. teissieri	KB115	This study	wRi
D. santomea	STO.2	Bom Successo, Africa	wSty
D. santomea	STO.7	Bom Successo, Africa	wSty
D. santomea	STO.9^e	Bom Successo, Africa	wSty
D. santomea	STO.4	Bom Successo, Africa	—
D. santomea	STO.8	Bom Successo, Africa	—
D. santomea	STO.10	Bom Successo, Africa	—
D. santomea	KB127	This study	wRi
D. santomea	KB128	This study	wRi

↵^a Based on partial wsp gene sequences.
↵^b Collected by Daniel Lachaise on São Tomé Island (Lachaise et al. 2000).
↵^c The D. yakuba strain 0261.0 was used as recipient to establish the two wRi-transinfected D. yakuba lines.
↵^d The D. teissieri strain 0257.0 was used as recipient, after tetracycline treatment, to establish the two wRi-transinfected D. teissieri lines.
↵^e The D. santomea strain STO.9 was used as recipient to establish the two wRi-transinfected D. santomea lines.
NDSRC, National Drosophila Species Resource Center.

TABLE 2

Naturally Wolbachia-infected D. yakuba, D. teissieri, and D. santomea and expression of CI

Cross (female × male)	Eggs	Crosses	% mortality	Comparison^a
1. D. yakuba (T) × D. yakuba (T)	1456	26	7.6 ± 1.7	1 vs. 2 (NS)
2. D. yakuba (T) × D. yakuba (wSty)	1660	26	9.3 ± 2.6
3. D. teissieri (T) × D. teissieri (T)	1424	26	13.9 ± 2.4	3 vs. 4 (NS)
4. D. teissieri (T) × D. teissieri (wSty)	1835	26	13.0 ± 2.6
5. D. santomea (T) × D. santomea (T)	807	19	8.8 ± 2.3	5 vs. 6 (NS)
6. D. santomea (T) × D. santomea (wSty)	1991	31	8.4 ± 2.3

↵^a Pairs of crosses were compared using the t-test.
CI is reported as percentage embryo mortality ±SE. Experiments for each Drosophila species were performed simultaneously. NS, no significant difference.

TABLE 3

wRi-transinfected D. yakuba, D. teissieri, and D. santomea and expression of CI

Cross (female × male)	Eggs	Crosses	% mortality	Comparison^a
1. D. yakuba (T) × D. yakuba (T)	1130	16	12.0 ± 5.9	1 vs. 2 (<0.0001)
2. D. yakuba (T) × D. yakuba (wRi)	2515	34	94.2 ± 0.8
3. D. teissieri (T) × D. teissieri (T)	617	16	14.3 ± 2.5	3 vs. 4 (<0.0001)
4. D. teissieri (T) × D. teissieri (wRi)	1421	32	82.4 ± 3.0
5. D. santomea (T) × D. santomea (T)	556	16	11.6 ± 2.9	5 vs. 6 (<0.0001)
6. D. santomea (T) × D. santomea (wRi)	1210	35	94.9 ± 1.3

↵^a Pairs of crosses were compared using the t-test. Values in parentheses are P-values.
CI is reported as percentage embryo mortality ±SE. Experiments for each Drosophila species were performed simultaneously. Crosses 2, 4, and 6 represent the pool of both transinfected lines of each species since there was no intraspecies difference between the lines.

TABLE 4

Compatibility relationships between naturally infected (wSty) and wRi-transinfected D. yakuba, D. teissieri, and D. santomea

Cross (female × male)	Eggs	Crosses	% mortality	Comparison^a
1. D. yakuba (T) × D. yakuba (wRi)	1247	17	95.1 ± 0.9	1 vs. 2 (<0.0001)
2. D. yakuba (wSty) × D. yakuba (wRi)	1321	16	36.9 ± 6.6	2 vs. 4 (NS)
3. D. yakuba (wRi) × D. yakuba (wRi)	1033	10	13.4 ± 5.9
4. D. yakuba (wSty) × D. yakuba (wSty)	1277	17	32.4 ± 4.9
5. D. teissieri (T) × D. teissieri (wRi)	802	17	88.0 ± 2.5	5 vs. 6 (<0.0001)
6. D. teissieri (wSty) × D. teissieri (wRi)	1314	16	26.2 ± 4.2	6 vs. 8 (NS)
7. D. teissieri (wRi) × D. teissieri (wRi)	1720	18	36.9 ± 4.8
8. D. teissieri (wSty) × D. teissieri (wSty)	847	12	22.2 ± 5.5
9. D. santomea (T) × D. santomea (wRi)	602	17	94.7 ± 1.8	9 vs. 10 (<0.0001)
10. D. santomea (wSty) × D. santomea (wRi)	1693	21	29.2 ± 3.3	10 vs. 12 (NS)
11. D. santomea (wRi) × D. santomea (wRi)	781	10	11.0 ± 3.3
12. D. santomea (wSty) × D. santomea (wSty)	873	11	35.7 ± 8.4

↵^a Pairs of crosses were compared using the t-test. Values in parentheses are P-values.
CI is reported as percentage embryo mortality ±SE. Experiments for each Drosophila species were performed simultaneously. NS, no significant difference.

TABLE 5
A purely quantitative interpretation of compatibility relationships among wMel, wRi, wAu, and wSty
mod resc
Wolbachia Quality^a Quantity^b Quality Quantity
wRi A + + + A + + +
wMel A + + A + +
wAu A − A −
wSty A − A + + +
↵^a Letters refer to qualitative variations of compatibility types.
↵^b + and − refer to quantitative variations (variations of Wolbachia density and/or variations of concentrations of the mod and resc factors).