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Rf8 and Rf* Mediate Unique T-urf13-Transcript Accumulation, Revealing a Conserved Motif Associated With RNA Processing and Restoration of Pollen Fertility in T-Cytoplasm Maize

Carren L. Dill, Roger P. Wise and Patrick S. Schnable
Genetics November 1, 1997 vol. 147 no. 3 1367-1379
Carren L. Dill
Interdepartmental Genetics Program, Iowa State University, Ames, Iowa 50011 Department of Plant Pathology, Iowa State University, Ames, Iowa 50011
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Roger P. Wise
Interdepartmental Genetics Program, Iowa State University, Ames, Iowa 50011 Department of Plant Pathology, Iowa State University, Ames, Iowa 50011 Corn Insects and Crop Genetics Research, U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS), Iowa State University, Ames, Iowa 50011
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Patrick S. Schnable
Interdepartmental Genetics Program, Iowa State University, Ames, Iowa 50011 Departments of Agronomy and Zoology and Genetics, Iowa State University, Ames, Iowa 50011
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Abstract

Rf8 is a newly described nuclear gene that can substitute for Rf1 to partially restore pollen fertility to male-sterile, T-cytoplasm maize. Families segregating for Rf8 were used to investigate the mechanism of this fertility restoration and to compare it to the restoration conditioned by Rf1. Although Rf8 is unlinked to the rf1 locus, it also alters T-urf13 mitochondrial transcript accumulation and reduces the accumulation of the URF13 protein. Like the 1.6- and 0.6-kilobase (kb) T-urf13 transcripts that accumulate in T-cytoplasm plants carrying Rf1, 1.42- and 0.42-kb transcripts accumulate in plants that are partially restored by Rf8. A survey of T-cytoplasm maize lines, inbreds, and F1 hybrids by mitochondrial RNA gel blot analyses revealed that Rf8, is rare in maize germplasm. These surveys revealed the presence of another rare, weak restorer factor, Rf*, which is uniquely associated with the accumulation of 1.4- and 0.4-kb T-urf13 transcripts. Primer extension analyses position the 5′ termini of the 1.42/0.42-kb and 1.4/0.4-kb transcripts at +137 and +159 nucleotides, respectively, 3′ of the AUG initiation codon of the T-urf13 reading frame. The conserved motif, 5′-CNACNNU-3′, overlaps the 5′ termini of the Rf1-, Rf8-, and Rf*-associated transcripts and the 380 nucleotide, Rf3-associated orf107 transcript from cytoplasmic male sterility sorghum. These results demonstrate that multiple unlinked, nuclear genes can have similar but distinct effects on the expression of the unique T-urf13 mitochondrial coding sequence to restore pollen fertility to T-cytoplasm maize.

  • Received December 19, 1996.
  • Accepted July 30, 1997.
  • Copyright © 1997 by the Genetics Society of America
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Volume 147 Issue 3, November 1997

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Rf8 and Rf* Mediate Unique T-urf13-Transcript Accumulation, Revealing a Conserved Motif Associated With RNA Processing and Restoration of Pollen Fertility in T-Cytoplasm Maize

Carren L. Dill, Roger P. Wise and Patrick S. Schnable
Genetics November 1, 1997 vol. 147 no. 3 1367-1379
Carren L. Dill
Interdepartmental Genetics Program, Iowa State University, Ames, Iowa 50011 Department of Plant Pathology, Iowa State University, Ames, Iowa 50011
  • Find this author on Google Scholar
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Roger P. Wise
Interdepartmental Genetics Program, Iowa State University, Ames, Iowa 50011 Department of Plant Pathology, Iowa State University, Ames, Iowa 50011 Corn Insects and Crop Genetics Research, U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS), Iowa State University, Ames, Iowa 50011
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Patrick S. Schnable
Interdepartmental Genetics Program, Iowa State University, Ames, Iowa 50011 Departments of Agronomy and Zoology and Genetics, Iowa State University, Ames, Iowa 50011
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  • Find this author on PubMed
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Citation

Rf8 and Rf* Mediate Unique T-urf13-Transcript Accumulation, Revealing a Conserved Motif Associated With RNA Processing and Restoration of Pollen Fertility in T-Cytoplasm Maize

Carren L. Dill, Roger P. Wise and Patrick S. Schnable
Genetics November 1, 1997 vol. 147 no. 3 1367-1379
Carren L. Dill
Interdepartmental Genetics Program, Iowa State University, Ames, Iowa 50011 Department of Plant Pathology, Iowa State University, Ames, Iowa 50011
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Roger P. Wise
Interdepartmental Genetics Program, Iowa State University, Ames, Iowa 50011 Department of Plant Pathology, Iowa State University, Ames, Iowa 50011 Corn Insects and Crop Genetics Research, U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS), Iowa State University, Ames, Iowa 50011
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Patrick S. Schnable
Interdepartmental Genetics Program, Iowa State University, Ames, Iowa 50011 Departments of Agronomy and Zoology and Genetics, Iowa State University, Ames, Iowa 50011
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site

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