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Senescence Mutants of Saccharomyces cerevisiae With a Defect in Telomere Replication Identify Three Additional EST Genes

Thomas S. Lendvay, Danna K. Morris, Jeannie Sah, Bhuvana Balasubramanian and Victoria Lundblad
Genetics December 1, 1996 vol. 144 no. 4 1399-1412
Thomas S. Lendvay
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030
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Danna K. Morris
Department of Cell and Molecular Biology, Baylor College of Medicine, Houston, Texas 77030
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Jeannie Sah
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030
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Bhuvana Balasubramanian
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030
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Victoria Lundblad
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030 Department of Cell and Molecular Biology, Baylor College of Medicine, Houston, Texas 77030
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Abstract

The primary determinant for telomere replication is the enzyme telomerase, responsible for elongating the G-rich strand of the telomere. The only component of this enzyme that has been identified in Saccharomyces cermzsiae is the TLC1 gene, encoding the telomerase RNA subunit. However, a yeast strain defective for the EST1 gene exhibits the same phenotypes (progressively shorter telomeres and a senescence phenotype) as a strain deleted for TLC1, suggesting that EST1 encodes either a component of telomerase or some other factor essential for telomerase function. We designed a multitiered screen that led to the isolation of 22 mutants that display the same phenotypes as est1 and tlc1 mutant strains. These mutations mapped to four complementation groups: the previously identified EST1 gene and three additional genes, called EST2, EST3 and EST4. Cloning of the EST2 gene demonstrated that it encodes a large, extremely basic novel protein with no motifs that provide clues as to function. Epistasis analysis indicated that the four EST genes function in the same pathway for telomere replication as defined by the TLC1 gene, suggesting that the EST genes encode either components of telomerase or factors that positively regulate telomerase activity.

  • Received June 25, 1996.
  • Accepted September 16, 1996.
  • Copyright © 1996 by the Genetics Society of America
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Volume 144 Issue 4, December 1996

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Senescence Mutants of Saccharomyces cerevisiae With a Defect in Telomere Replication Identify Three Additional EST Genes

Thomas S. Lendvay, Danna K. Morris, Jeannie Sah, Bhuvana Balasubramanian and Victoria Lundblad
Genetics December 1, 1996 vol. 144 no. 4 1399-1412
Thomas S. Lendvay
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Danna K. Morris
Department of Cell and Molecular Biology, Baylor College of Medicine, Houston, Texas 77030
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jeannie Sah
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Bhuvana Balasubramanian
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Victoria Lundblad
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030 Department of Cell and Molecular Biology, Baylor College of Medicine, Houston, Texas 77030
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  • Find this author on PubMed
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Citation

Senescence Mutants of Saccharomyces cerevisiae With a Defect in Telomere Replication Identify Three Additional EST Genes

Thomas S. Lendvay, Danna K. Morris, Jeannie Sah, Bhuvana Balasubramanian and Victoria Lundblad
Genetics December 1, 1996 vol. 144 no. 4 1399-1412
Thomas S. Lendvay
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Danna K. Morris
Department of Cell and Molecular Biology, Baylor College of Medicine, Houston, Texas 77030
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jeannie Sah
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Bhuvana Balasubramanian
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Victoria Lundblad
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030 Department of Cell and Molecular Biology, Baylor College of Medicine, Houston, Texas 77030
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site

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