15 Results
for term "sites"
- Identification of Virulence Mutants of the Fungal Pathogen Cryptococcus neoformans Using Signature-Tagged Mutagenesis...crossover event, creating a duplication of the actin promoter and the integration of single or multiple copies of the vector. Analysis of the chromosomal insertion sites of those mutants that did not have an integration event in the actin promoter revealed an approximately random distribution among ~~~
- An STE12 Homolog From the Asexual, Dimorphic Fungus Penicillium marneffei Complements the Defect in Sexual Development of an Aspergillus nidulans steA Mutant.... (1989) pRS31 gpdA(p)::stuA::gfp Suelmann et al. (1997) pAmPh520 Tn5 ble R gene expressed from the N. crassa am promoter Austin et al. (1990) pAB4342 1.4-kb XhoI-BamHI fragment of pJR15 end filled with This study Klenow polymerase and inserted into the SmaI site of pBluescript II SK1 pAB4458 3.4-kb Eco ~~~
- The ste3 Pheromone Receptor Gene of Pneumocystis carinii Is Surrounded by a Cluster of Signal Transduction Genes...DNA was generated by PCR amplification of the longest ste3 cDNA cloneGold (Molecular Probes, Eugene, OR) and visualized under UV light. DNA in an agarose block was purified using using a primer located at the predicted translational start and within the vector 39 to the insert cloning site, and the full ~~~
- The Neurospora crassa Genome: Cosmid Libraries Sorted by Chromosome...HI-underway at University of New Mexico (UNM; Nelson et al. XhoI linker oligonucleotide (59-GATCCCTCGAGG-39) to gen-1997), University of Oklahoma (OU), and Dartmouth Medical erate an intermediate plasmid pLorist6X, which, using theSchool (DMS; Zhu et al. 2001). Cosmid end-sequencing of half-site fill-in method (Zabarovsky ~~~
- Mapping of Avirulence Genes in Phytophthora infestans With Amplified Fragment Length Polymorphism Markers Selected by Bulked Segregant Analysis...were observed at different inoculation sites the number in each classNomenclature of genes and phenotypes: Anticipating that the six avirulence genes analyzed in this study would be domi- was counted. DNA isolation and AFLP DNA fingerprinting: DNA isolationnant, the nomenclature used for these genes ~~~
- Characterization of the Aspergillus nidulans Septin (asp) Gene Family...organization pro- Cdc11p, and Cdc12p. In addition to their location at thecesses ranging from cytokinesis to surface growth. neck, Cdc3p, Cdc10p, Cdc11p, and Cdc12p are found at Members of the septin gene family have been found in the site of bud emergence and at the base of the mating budding and fission ~~~
- ODS2: A Multiplatform Software Application for Creating Integrated Physical and Genetic Maps...for sequence-tagged sites (STSs), and direct et al. 1995). Clone chimerism is a problem associated detection of clone overlaps. Clones may be fingerprinted with all mapping protocols. Chimerism is especially by treatment with restriction enzymes and measurement common with certain cloning vectors ~~~
- Hybridization-Based Mapping of Neurospora crassa Linkage Groups II and V...- contained only genomic fragments that were isolated by restriction digestion of genomic DNA. At least, theduced in the end. This and the fact that independently produced libraries of high clone coverage were analyzed DNA beyond the most telomeric restriction sites cannot therefore be present ~~~
- Analysis of Expressed Sequence Tags From Two Starvation, Time-of-Day-Specific Libraries of Neurospora crassa Reveals Novel Clock-Controlled Genes...the website http://www.genome.ou.edu/fungal.html. excision converted the single-stranded phagemids to the dou- The site also provides the opportunity to search the EST seble-stranded form. The appropriate volume of F1 lysate con- quences using BLAST. The EST sequences were also deposited taining the single ~~~