9 Results
for term "sites"
- Genomic Rearrangements at rrn Operons in Salmonella...by homologous recomS. enterica serovar Typhi, which infects only humans, and bination at another site (Figure 2B). Both of these S. enterica serovar Pullorum, which causes disease only events result in the reorganization of large fragments in fowl. These host-specific serovars cause a systemic of the chromosome ~~~
- Evolution in Saccharomyces cerevisiae: Identification of Mutations Increasing Fitness in Laboratory Populations...noncoding region of FAR3, at#5;35 relative to the transla-Relative fitnesses estimated from reconstruction experiments tion start site and in the same transcriptional orientation (Figure 1). Upstream from FAR3 is a tRNA-Trp gene andNo. of Fitness SE relative Strain replicates to wild type (unity)a several ~~~
- Activity of Mitochondrially Synthesized Reporter Proteins Is Lower Than That of Imported Proteins and Is Increased by Lowering cAMP in Glucose-Grown Saccharomyces cerevisiae Cells...from pXP2 containing TPK1 into the BamHI1973; Fox et al. 1991). After mutagenesis, cells were grown in site of pRS315 (Sikorski and Hieter 1989). pTD46 was madeYPD to early log phase and Arg auxotrophs were enriched by subcloning the XbaI-SstI fragment containing TPK2 fromfor by inositol starvation ~~~
- A Method for Gene Disruption That Allows Repeated Use of URA3 Selection in the Construction of Multiply Disrupted Yeast Strains...that if such a construct were introduced into a cloned gene and integrated into the yeast genome, it would undergo frequent mitotic recombination between the direct repeats to eliminate the URA3 gene and leave behind a single copy of the repeat sequence at the site of the original integration (Figure 1). Mitotic ~~~
- Mutations in New Cell Cycle Genes That Fail to Complement a Multiply Mutant Third Chromosome of Drosophila...of Anatomy and Physiology, Medical Sciences Institute, University of Dundee, Dundee DD1 4HN, Scotland Manuscript received November 21, 1995 Accepted for publication August 3, 1996 ABSTRACT We have simultaneously screened for new alleles and second site mutations that fail to complement five cell cycle ~~~
- A Role for GEA1 and GEA2 in the Organization of the Actin Cytoskeleton in Saccharomyces cerevisiae...by fluores-PFY1 and the RHO2 genes (pfy1-111 rho2#1;). The GEA1 cence staining with 1 m FITC-conjugated phalloidin.and GEA2 genes were identified as suppressors of the The coding sequences of ARF1, ARF2, and ARF3 were in-mutant phenotype. Gea1p and Gea2p have partially overserted into the BamHI site ~~~
Figure 2.—Two different ways chromosomal rearrangements can occur. (A) Two regions of homology in opposite orientation can recombine to yield an inversion in the chromosome and two hybrid rrn operons. (B) Regions of homology in direct orientation can recombine to generate a transient circular DNA fragment that can subsequently recombine with the chromosome at a different site, yielding a levitation of the rrn interval and three hybrid rrn operons.
Figure 1.— Ty1 insertion upstream of FAR3, in clone F isolated from the long-term serial dilution population. Structure of chromosome VIII in S288c in the FAR3 region and Ty1 insertion in clone F. Ty1 insertion is not to scale. Arrows indicate direction of transcription. The nucleotide sequence of the FAR3 fragment generated from inverse PCR is compared to the same region in S288c. Translation start sites in the two sequences are in bold-face type, and the NlaIII sites are underlined. Thick arrows indicate direction of transcription and thin arrows show primer binding sites for PCR amplification of the Ty1::far3 allele. δ, δ elements.
Figure 2.—Ty1 insertion into FAR3 in the clone isolated from population I, genetically variable for Ty1 insertions (see text for details). Insertion occurs after nucleotide 180. The amino acid sequence of FAR3 translated from the DNA sequence is shown below the diagram. The sequence includes the site of Ty1 insertion, as well as part of the Ty1 sequence, shown in boldface type. Asterisks represent stop codons.