215 Results
for term "sites"
- Generating Customized Transgene Landing Sites and Multi-Transgene Arrays in Drosophila Using phiC31 Integrase...GENETICS | INVESTIGATION Jon-Michael Knapp,1 Phuong Chung, and Julie H. Simpson1 Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia 20147 ABSTRACT Transgenesis in numerous eukaryotes has been facilitated by the use of site-specic integrases to stably insert transgenes ~~~
- Scalable and Versatile Genome Editing Using Linear DNAs with Microhomology to Cas9 Sites in Caenorhabditis elegans...to antigenic peptides and GFP. Whole-genome sequencing of ve edited strains revealed the presence of passenger variants, but no mutations at predicted off-target sites. The method is scalable for multi-gene editing projects and could be applied to other animals with an accessible germline. THE ultimate goal ~~~
- Large-Scale Mapping of Transposable Element Insertion Sites Using Digital Encoding of Sample Identity.... 2009; Goodman et al. 2009; Prabhu and Peer 2009). Transposable elements represent powerful tools for manipulating the genomes of many model organisms (Bellen et al. 2011; Bire and Rouleux-Bonnin 2012). Thus, determining the genomic location of transposon insertion sites is a common experimental goal ~~~
- Whole-Genome Restriction Mapping by “Subhaploid”-Based RAD Sequencing: An Efficient and Flexible Approach for Physical Mapping and Genome Scaffolding...by generating hundreds of subhaploid fosmid/ bacterial-articial-chromosome clone pools, and by restriction site-associated DNA sequencing of these clone pools to produce an ultradense whole-genome restriction map to facilitate genome scaffolding. A bootstrap-based minimum spanning tree algorithm is developed ~~~
- GeneImp: Fast Imputation to Large Reference Panels Using Genotype Likelihoods from Ultralow Coverage Sequencing...are recorded in the majority of sites, rather than ultralow coverage data, where many sites have no reads. In the latter case, MarViNs Expectation-Maximization algorithm is likely to take considerably more iterations to converge. Moreover, as reported in Arthur et al. (2015), its homogeneity assumption ~~~
- Alternative Polyadenylation Directs Tissue-Specific miRNA Targeting in Caenorhabditis elegans Somatic Tissues...transcriptomes of intestine, pharynx, and body muscle tissues, precisely assigns tissue-specic expression dynamics for 60% of all annotated C. elegans protein-coding genes, providing an important resource for the scientic community. The mapping of 15,956 unique high-quality tissue-specic polyA sites in all ~~~
- Tools for Targeted Genome Engineering of Established Drosophila Cell Lines...mediated targeted cassette exchange for use in Drosophila cell lines. Single copies of an attP-bounded docking platform carrying a GFP-expression marker, with or without insulator elements anking the attP sites, were inserted by P-element transformation into the Kc167 and Sg4 cell lines; each of the resulting ~~~
- Targeted Integration of Single-Copy Transgenes in Drosophila melanogaster Tissue-Culture Cells Using Recombination-Mediated Cassette Exchange...we developed a system for Drosophila cell lines that allows selection of cells with a single-copy transgene inserted at a specic genomic site using recombination-mediated cassette exchange (RMCE). We used the fC31 integrase and its target sites attP and attB for RMCE. Cell lines with an att ~~~
- Second-Generation Drosophila Chemical Tags: Sensitivity, Versatility, and Speed...al. (2014) and the new reporters from this study. (B) Labeling of Mz19Gal4 neurons using the old and new reporters. Each panel contains information on the dye used and insertion sites. Dotted boxes in images highlight the Lateral Horn region used for quantication. Boxplots show the quantication ~~~
- TargetOrtho: A Phylogenetic Footprinting Tool to Identify Transcription Factor Targets...of transcription factors is central to our understanding of how transcription factors fulll their many key roles in development and homeostasis. DNA-binding sites have been uncovered for many transcription factors through a number of experimental approaches, but it has proven difcult to use this binding site ~~~