10 Results
for term "sites"
- A 2600-Locus Chromosome Bin Map of Wheat Homoeologous Group 2 Reveals Interstitial Gene-Rich Islands and Colinearity With Rice...to have significant colinearity were assigned to putative orders within bins on theother factors such as intragenic restriction sites. These factors confound the analysis of duplication of genetic basis of comparison with the ordered rice BAC/PAC positions along the rice chromosomes (Figure 3; supple ~~~
- Chromosome Bin Map of Expressed Sequence Tags in Homoeologous Group 1 of Hexaploid Wheat and Homoeology With Rice and Arabidopsis...of those bins and not curring in 1B (Table 1). Approximately 13% of the mapped EST unigenes contained at least one EcoRI re-at the ends of the chromosomes. The consensus map with a greater number of long-arm bins clearly points striction site, not including sites within introns (Munkvold et al. 2004 ~~~
- Construction and Evaluation of cDNA Libraries for Large-Scale Expressed Sequence Tag Sequencing in Wheat (Triticum aestivum L.)...I and Not Idancy with sequences from other libraries. More complete cloning sites in the vector pSPORT1 and the Escherichia colidetails on the composition of each library listed in Table 1 host strain DH12S. Library SC013XXX was produced by G. E.can be viewed using the HarvEST:Wheat software (http ~~~
- A Chromosome Bin Map of 16,000 Expressed Sequence Tag Loci and Distribution of Genes Among the Three Genomes of Polyploid Wheat...al. 2004; BE442924, a B-genome-specific EST unigene, at the web Hossain et al. 2004; Linkiewicz et al. 2004; Miftahudin et site (http://wheat.pw.usda.gov/cgi-bin/westsql/map_ al. 2004; Munkvold et al. 2004; Peng et al. 2004; Randhawa locus.cgi).et al. 2004). This is because analysis by genome differs ~~~
- Group 3 Chromosome Bin Maps of Wheat and Their Relationship to Rice Chromosome 1...betweention fragments would be due to within-gene restriction adjacent consensus bins, numerous single ESTs matchedsites. Approximately 13% of the mapped-EST unigenes sequences on the other rice chromosomes. In fact, thecontained at least one EcoRI restriction site, not includwheat homoeologous group 3 ~~~
- Development of an Expressed Sequence Tag (EST) Resource for Wheat (Triticum aestivum L.)...site and purified using QIAQuick 96 kits (QIAGEN, Chatsworth, CA) either named 0064-0067 to indicate that, when expanded to 96589ESTs for Wheat Physical Mapping TABLE 2 Sequence editing steps and nomenclature involved in trimming DNA sequence files Step Sequence nomenclature 1. Shred (SQPR) 0064_H10 ~~~
- Analysis of Expressed Sequence Tag Loci on Wheat Chromosome Group 4...-square test indicates the probability of a departure from the expected loci. produced. These images were then uploaded to the NSF web- 0.86-1.00. For example, wheat EST BE497446 mapped to deletion bins 4AL12-0.43-0.59, 4BS8-0.57-0.81, and 4DS3-0.67-site and scored again by a homoeologous group coordinator ~~~
- A 2500-Locus Bin Map of Wheat Homoeologous Group 5 Provides Insights on Gene Distribution and Colinearity With Rice...is an overestimate, because internal cal tests of colinearity (Table 3). Hereafter, wheat chromosomes are preceded by a W and rice chromosomesEcoRI sites within the region of hybridization also can create duplicated fragments within a bin. by an R to simplify the descriptions. In the short arm, most of the ESTs (64 ~~~
- A Chromosome Bin Map of 2148 Expressed Sequence Tag Loci of Wheat Homoeologous Group 7....1 BE445506 0.901.00 AP004685 C607 6 124.6 a The intervals of each consensus chromosome arm are presented from proximal to distal. Duplication: There are duplicated loci (paralogous) loci in the same deletion bin (intrabin) and these could have resulted from the internal cut site of EcoRI withinon ~~~
Figure 3.—Depiction of wheat consensus group 2 and rice syntenous blocks. Wheat group 2 ESTs are listed in their putative order within consensus bins on the basis of comparison with ordered rice BAC/PAC clones. The wheat EST consensus map showing loci with matches to the rice colinear chromosomes is flanked by the genetic maps of rice BAC/PAC clones for rice chromosomes 4 (left) and 7 (right). Solid lines connect syntenous blocks between wheat ESTs and rice BAC/PAC clones. Dotted lines indicate significant blast matches between a wheat EST and rice BAC/PAC showing synteny disruptions at the consensus bin level. ESTs involved in synteny disruptions are shown in boldface, underlined type. Thin solid lines connect EST-rice BAC/PAC matches that are syntenous at the bin level, but have only one match to the syntenous region in rice. Centromeres are indicated by thick solid horizontal lines. Cumulative centimorgan distances are given on the side of each rice chromosome. Information on genetic mapping of rice chromosomes 4 and 7 BAC/PAC clones was obtained at http://www.tigr.org/tigr-scripts/IRGSP/assignMap.pl?chr=4&site=All&markerSource=All and http://www.tigr.org/tigr-scripts/IRGSP/assignMap.pl?chr=7&site=All&markerSource=All.