17 Results
for term "sites"
- A Multifunctional Mutagenesis System for Analysis of Gene Function in Zebrafish...). These mutants are valuable tools for the study of their human orthologs. Protein trapping offers an alternative, powerful approach to abolish gene function by random insertion of DNA. A protein trap construct typically contains a splice acceptor site immediately upstream of a promoter-less reporter gene ~~~
- A Genetic Screen To Assess Dopamine Receptor (DopR1) Dependent Sleep Regulation in Drosophila...of a recombination of control fly line that is empty at the recombination attp2 site at 68A4 on 3L ( Jenett et al. 2012 ; Pfeiffer et al. 2008 ). Controls for Gal4 lines of the Kaiser collection ( Armstrong et al. 1998 ) were performed by crossing CS virgins flies to males with the Gal4 enhancer trap c561, which ~~~
- An RNAi Screen To Identify Protein Phosphatases That Function Within the Drosophila Circadian Clock...for deletions, a600 bp DNA fragment containing the gRNA binding sites was amplied using primers 59TGACCAAAGGGCGAGATTAG-39 and 59-ACATAGTCGCCCAG GAACAG-39 via PCR, and sequenced. In a screen of 150 injected adults, three independent deletion mutants were recovered (Figure 1). Testing for the presence of per ~~~
- Rapid and Efficient Identification of Caenorhabditis elegans Legacy Mutations Using Hawaiian SNP-Based Mapping and Whole-Genome Sequencing...suppressor screens. Suppressor screens allow for an unbiased approach in the identication of second-site extragenic mutations that ameliorate the phenotype of the original mutation. The use of temperature-sensitive alleles has the advantage of being able to perform mutagenesis on homozygous lines ~~~
- Novel Interactome of Saccharomyces cerevisiae Myosin Type II Identified by a Modified Integrated Membrane Yeast Two-Hybrid (iMYTH) Screen..., and possibly serves as a site for the recruitment of the cytokinesis machinery and/or to signal for cell division (Lister et al. 2006). Previous yeast two-hybrid experiments (Drees et al. 2001; Wang et al. 2012), and TAP-tag protein copurication experiments (Gavin et al. 2002) identied multiple putative Myo1 ~~~
- A Genetic Screen Based on in Vivo RNA Imaging Reveals Centrosome-Independent Mechanisms for Localizing gurken Transcripts in Drosophila...localization, suggesting that microtubule self-organization contributes to breaking axial symmetry to generate a unique dorsoventral axis. KEYWORDS oogenesis RNA localization microtubules whole-genome sequencing mutant mapping Asymmetric messenger (m)RNA localization in the cytoplasm acts to restrict the sites ~~~
- A Forward Genetic Screen for Molecules Involved in Pheromone-Induced Dauer Formation in Caenorhabditis elegans...Grath et al. 2011; Park et al. 2012), whereas ascr#2 and ascr#3 (C9, asc-DC9, daumone 3) are also detected by the SRBC-64/SRBC-66 GPCRs in the ASK chemosensory neurons that regulate dauer formation (Kim et al. 2009) (Figure 1A). Sites of detection of other ascarosides in the dauer decision are largely ~~~
- par-1, Atypical pkc, and PP2A/B55 sur-6 Are Implicated in the Regulation of Exocyst-Mediated Membrane Trafficking in Caenorhabditis elegans..., it has been shown that insulin-stimulated GLUT4 storage vesicle exocytosis is regulated by exocyst complex function and involves the Ser/Thr kinase AKT (Stockli et al. 2011). Analysis of phosphopeptides in several model systems has identied potential in vivo phosphorylation sites in the exocyst subunits ~~~
- Leveraging DNA Damage Response Signaling to Identify Yeast Genes Controlling Genome Stability.... Coates, and S. P. Jackson, 2005 Conserved modes of recruitment of ATM, ATR and DNA-PKcs to sites of DNA damage. Nature 434: 605611. 1004 | J. A. Hendry et al. Forbes, S. A., D. Beare, P. Gunasekaran, K. Leung, N. Bindal et al., 2015 COSMIC: exploring the worlds knowledge of somatic mutations in human ~~~
- Saccharomyces cerevisiae Essential Genes with an Opi− Phenotype..., 2005 A highly conserved binding site in vesicle-associated membrane protein-associated protein (VAP) for the FFAT motif of lipid-binding proteins. J. Biol. Chem. 280: 1409714104. 766 | B. Salas-Santiago and J. M. Lopes Loewen, C. J., A. Roy, and T. P. Levine, 2003 A conserved ER targeting motif ~~~