35 Results
for term "sites"
- Neurons That Underlie Drosophila melanogaster Reproductive Behaviors: Detection of a Large Male-Bias in Gene Expression in fruitless-Expressing Neurons..., Table S1 and Table S2). Motif analysis MAST (Bailey and Gribskov 1998) uses a le of motifs (FruA, B, and C DNA binding motifs determined using SELEX), and a sequence FASTA le to identify Fru binding sites in a region that includes the gene of interest, 2 kb upstream of the transcription start site ~~~
- Signatures of Sex-Antagonistic Selection on Recombining Sex Chromosomes...of selection. Linkage disequilibrium between neutral sites is also inated. We show how the pattern of divergence is altered when the SA polymorphism or the sex-determining region was recently established. We use data from the owering plant Silene latifolia to illustrate how the strength of SA selection might ~~~
- Comparative Genomics of the Ectomycorrhizal Sister Species Rhizopogon vinicolor and Rhizopogon vesiculosus (Basidiomycota: Boletales) Reveals a Divergence of the Mating Type B Locus...site located on Marys Peak in the Oregon Coast Range. Coordinates and ecological properties of this site are detailed in Dunham et al. (2013). Each sporocarp was cleaned of adhering debris using a damp cloth, and divided into two hemispheres using a ame sterilized scalpel. Small sections (1 mm3 ~~~
- Cell-Specific mRNA Profiling of the Caenorhabditis elegans Somatic Gonadal Precursor Cells Identifies Suites of Sex-Biased and Gonad-Enriched Transcripts...were made to determine signicant overlap between sex-biased transcripts and genes near TRA-1 binding sites (P , 0.05) as well as to determine whether sex-biased or gonad-enriched transcripts were signicantly over-represented on a chromosome. (P values of, 0.01 and 0.001 are indicated ~~~
- An Unusual Role for doublesex in Sex Determination in the Dipteran Sciara...designed to allow the joining of the four fragments in the order E1I1 I1E2I2I2E3I3I3E4 and this construct was cloned in the pUAST vector. To do this, the primers contained restriction enzyme sites: e1i1-2 and i1e2i1-1 contained the XbaI sequence, i1e2i2-2 and i2e3i3-1 contained the PstI sequence, i2e3i3 ~~~
- Plasticity in the Meiotic Epigenetic Landscape of Sex Chromosomes in Caenorhabditis Species...interspaced short palindromic repeats/Cas9 generation of Cbr-met-2 mutants Cbr-met-2 targeting sequences were inserted into the Cas9 + single guide RNA (sgRNA) vector pDD162 (Peft-3::Cas9 + Empty sgRNA) using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs, Beverely, MA) with forward primer 59-N19 ~~~
- Advances in Understanding Mating Type Gene Organization in the Mushroom-Forming Fungus Flammulina velutipes...substitution model was selected, assuming an estimated proportion of invariant sites and four gamma-distributed rate categories to account for rate heterogeneity across sites. The gamma shape parameter was estimated directly from the data. Reliability of internal branches was assessed using LRT testing (SH ~~~
- Turnover of Sex Chromosomes in Celebensis Group Medaka Fishes...-specic mutation sites found in theO. marmoratus SD region. Association analysis We obtained wild O. marmoratus (N = 68) and O. profundicola (N = 34) stocks from Shinshu University and the National Institute for Basic Biology. We genotyped 10 Y-specic mutation regions conserved between the O. marmoratus and O ~~~
- Self-Incompatibility in Brassicaceae: Identification and Characterization of SRK-Like Sequences Linked to the S-Locus in the Tribe Biscutelleae...developed a method to minimize amplication of this gene before the cloning step intended to obtain SRK sequences. We identied a restriction site that was present only in this sequence and digested the DNA (puried PCR product) with the HindIII restriction enzyme (A-AGCTT). The restriction was carried out ~~~
- The Evolving Puzzle of Autosomal Versus Y-linked Male Determination in Musca domestica...occupies a denite site on the respective chromosomes (Inoue et al. 1983). The authors concluded that AM factors are located in centric heterochromatin on each autosome and M factors on a given chromosome are all at the same locus. Alternatively, it was proposed that the M factors on the different ~~~