18 Results
for term "sites"
- Efficient CRISPR/Cas9-Mediated Genome Editing in Mice by Zygote Electroporation of Nuclease...naturally occurring RNA species, CRISPR RNA (crRNA) and trans-activating CRISPR RNA (tracrRNA). This complex targets specic DNA sequences complementary to the 20-nt sequence residing at the 59 end of the crRNA and generate DNA double stranded breaks (DSBs) at the target site (Jinek et al. 2012 ~~~
- In Vivo Transcriptional Activation Using CRISPR/Cas9 in Drosophila...active chimeric activator domain. In this study, we characterize the dCas9-VPR system in Drosophila cells and in vivo. We show that this system can be used in cell culture to upregulate a range of target genes, singly and in multiplex, and that a single guide RNA upstream of the transcription start site ~~~
- Pharmacological Inhibition of the DNA Damage Checkpoint Prevents Radiation-Induced Oocyte Death...(total of 6 ovaries per recipient female). The females were allowed a recovery period of 6 weeks, then housed with males. Three to four months later, upon euthanasia, dissection was performed for visual inspection of the transplantation sites. Whole organ immunofluorescence Ovaries cultured for seven ~~~
- A Novel Approach for Studying Histone H1 Function in Vivo...expression and its association with chromatin in vivo. We show that histone H1 is subject to negative autoregulation and exploit this result to examine the effects of mutations of the main phosphorylation site of histone H1. KEYWORDS Drosophila; histone H1; chromatin; gene expression HISTONE H1 is a key ~~~
- A Surprising Role for the Sch9 Protein Kinase in Chromosome Segregation in Candida albicans...longevity only under normoxic conditions and not under hypoxic conditions (Stichternoth et al. 2011). In this study, we sought to determine the genomic binding sites of the HA-tagged Sch9 protein by ChIP on chip (ChIPchip) experiments under normoxia as well as hypoxia with and without elevated CO2 levels ~~~
- Modeling the Manipulation of Natural Populations by the Mutagenic Chain Reaction...al. 2014; Oye et al. 2014). Another possibility is that genetic variation for conversion susceptibilitymay segregate in populations. It is likely that the target site of the guide RNA would be variable in natural populations. In this case, some naturally occurring wild-type alleles would both ~~~
- Synaptonemal Complex Components Are Required for Meiotic Checkpoint Function in Caenorhabditis elegans...-acting sites that promote pairing and synapsis. We have hypothesized that the stability of homolog pairing at PCs is monitored by this checkpoint. Here, we report that SC components SYP-3, HTP-3, HIM-3, and HTP-1 are required for a functional synapsis checkpoint. Mutation of these components does not abolish ~~~
- Sgs1 and Mph1 Helicases Enforce the Recombination Execution Checkpoint During DNA Double-Strand Break Repair in Saccharomyces cerevisiae..., strand invasion by the homologous end results in the establishment of a migrating D-loop that can copy all sequences distal to the site of homology, resulting in a nonreciprocal translocation (Donnianni and Symington 2013; Saini et al. 2013; Wilson et al. 2013). Sequences on the other side of the break ~~~
- Massively Parallel Functional Analysis of BRCA1 RING Domain Variants...(26 126)GSBRCA1(2304) fusion open reading frame (ORF) (Christensen et al. 2007) was moved to the pGEM vector and the EcoRI site in BRCA1 was destroyed. This fragment of BRCA1 was used as a template for PALS mutagenesis (Kitzman et al. 2015). Sixteen base random barcodes (16N) were added 39 of the stop ~~~
- Dual Color Neural Activation and Behavior Control with Chrimson and CoChR in Caenorhabditis elegans...clones through gene synthesis (Genewiz, South Plaineld, NJ). The clones were created in the pUC57-kan backbone and were anked with attL1 and attL2 recombination sites to be used directly as ENTRY vectors in the Multisite Gateway Cloning System (Invitrogen). These Entry clones have been submitted ~~~