25 Results
for term "sites"
- A Bitter Taste of the Sun Makes Egg-Laying Flies Run..., evidence of diverse behavioral roles for bitter GRNs in feeding aversion, aggression, courtship inhibition, positional avoidance, and egg-laying site selection (Marella et al. 2006; Miyamoto and Amrein 2008; Koganezawa et al. 2010; Wang et al. 2011; Weiss et al. 2011; Joseph and Heberlein 2012; Charlu et ~~~
- Taking Stock of the Drosophila Research Ecosystem...(below) (Venken et al. 2011; Nagarkar-Jaiswal et al. 2015). Past efforts took advantage of random insertions, but new initiatives will use CRISPR/Cas9-mediated site-specic targeting to extend the Drosophila mutant kit (Zhang et al. 2014; Diao et al. 2015). Targeted gene manipulation: The ability ~~~
- CRISPR/Cas9 Gene Drive: Growing Pains for a New Technology...-bp nucleotide sequence in the genome (see for review Ran et al. 2013). Individuals can be resistant to CGD if they have an alternative nucleotide sequence at the target site. Unckless et al. (2017) address this issue by considering the likelihood of both standing variation in the population and new ~~~
- Maize Transposon Storm Kicks up a White Cap...of the hAT TE family. The story begins with a shower of Tam3L insertions in and around the vicinity of the progenitor Ccd1 locus, producing a macrotransposon composed of Tam3L elements anking Ccd1 and two other genes. Transposition of the macrotransposon to a nearby site generated the incipient Wc locus ~~~
- Alternative Poly(A) Tails Meet miRNA Targeting in Caenorhabditis elegans...,956 unique, high-quality tissue-specic poly(A) sites in all eight somatic tissues revealed a pervasive tissue-specic 39UTR isoform switching through APA. In addition to widespread occurrences of APA, the obtained information suggests that APA may be a signicant regulatory mechanism to ne-tune tissue ~~~
- Targeted Gene Replacement in Drosophila Goes the Distance...for gene targeting in Drosophila. These methods, which allow for rapid replacement of large regions adjacent to existing transgenic insertions, are described in two articles published in this months GENETICS: Long-Range Targeted Manipulation of the Drosophila Genome by SiteSpecic Integration ~~~
- Freedom and Responsibility in Synthetic Genomics: The Synthetic Yeast Project.... These engineered features will be used as tools to answer a number of age-old biological questions. Two of these genome-wide genetic tools are (1) symmetrical loxP sites, introduced throughout the genome, which will be used in genome structure/evolution experiments, and (2) the swapping out of all TAG with TAA ~~~
- Disproportionate Roles for the X Chromosome and Proteins in Adaptive Evolution...tested repeatedly by comparing between-species divergence at X-linked and autosomal genes (Meisel and Connallon 2013). The most popular approach calculates the relative rate of substitution at nonsynonymous and synonymous sites, reasoning that nonsynonymous changes will often be targeted by selection ~~~
- To Pool, or Not to Pool?...propose a minimum required allele frequency for inclusion of a site in SNP-based analyses. Based on the assumption that sequencing errors will be rare if this minimum frequency is sufficiently large, this approach resigns itself to the loss of a tremendous amount of informationnotable particularly given ~~~
- Dense Core Vesicle Release: Controlling the Where as Well as the When...they bud from the Golgi in the soma and before they are trafcked to their release sites in the axon. This role of CaMKII requires it to have kinase activity as well as an activating calcium signal released from internal ER stores via the ryanodine receptor. Not only does this represent a novel function ~~~