249 Results
for term "sites"
- DNA Replication Stress Phosphoproteome Profiles Reveal Novel Functional Phosphorylation Sites on Xrs2 in Saccharomyces cerevisiae...| INVESTIGATION DNA Replication Stress Phosphoproteome Proles Reveal Novel Functional Phosphorylation Sites on Xrs2 in Saccharomyces cerevisiae Dongqing Huang, Brian D. Piening,1 Jacob J. Kennedy, Chenwei Lin, Corey W. Jones-Weinert, Ping Yan, and Amanda G. Paulovich2 Fred Hutchinson Cancer ~~~
- Traffic Through the Trans-Golgi Network and the Endosomal System Requires Collaboration Between Exomer and Clathrin Adaptors in Fission Yeast...deletions were generated by replacing the gene ORF by the KANMX6 andNATMX6 selection markers from cassettes bearing 0.5 kb DNA sequences upstream and downstream from the ORF. The Green Fluorescent Protein (GFP)was introduced as aNotI-NotI DNA fragment at NotI sites created by site-directed mutagenesis ~~~
- Editing of Mitochondrial Transcripts nad3 and cox2 by Dek10 Is Essential for Mitochondrial Function and Maize Plant Development...10 is responsible for the C-to-U editing at nad3-61, nad3-62, and cox2-550 sites, which are specic editing sites in monocots. The defects of these editing sites result in signicant reduction of Nad3 and the loss of Cox2. Interestingly, the assembly of complex I was not reduced, but its NADH ~~~
- Differential Phosphorylation Provides a Switch to Control How α-Arrestin Rod1 Down-regulates Mating Pheromone Response in Saccharomyces cerevisiae...identied two stress-responsive protein kinases that phosphorylate Rod1 in vivo and delineated the sites at which they exert their regulatory effect. Our studies also reveal that, in the absence of its phosphorylation, Rod1 can, like Rog3, also promote adaptation in an Rsp5independent manner, suggesting ~~~
- Nutritional Control of Chronological Aging and Heterochromatin in Saccharomyces cerevisiae...(cre-EBD), from daughter-cell-specic promoter pSCW11. Elsewhere in the genome, loxP sites ank a red uorescent protein (RFP) upstream of a promoterless GFP. When cre-EBD is produced in a newly forming daughter cell in the presence of estradiol, cells switch from transcribing RFP to transcribing GFP ~~~
- Involvement of Conserved Amino Acids in the C-Terminal Region of LINE-1 ORF2p in Retrotransposition...there is some data concerning the TPRT process from the L1 ORF2p (Feng et al. 1996). The R2 protein is organizationally different from the L1 ORF2p. From the N to the C terminus, this molecule contains a DNA-binding domain that confers target site specicity (Xiong and Eickbush 1988; Luan et al. 1993), a Z ~~~
- A Genetic Analysis of the Caenorhabditis elegans Detoxification Response.... Structures of the highly homologous mouse and human ALDH4A1 proteins localized the binding sites for NAD and glutamate to this domain (Srivastava et al. 2012). Mutations that we (Figure 1A, in blue) and others (in black) have identied in alh-6a are adjacent to the active site residues that contact glutamate ~~~
- New Regulators of Clathrin-Mediated Endocytosis Identified in Saccharomyces cerevisiae by Systematic Quantitative Fluorescence Microscopy...of Lucifer Yellow and the methionine transporter Mup1 demonstrating that Ubx3 is needed for efcient endocytosis. Further, the UBX domain is required both for localization and function of Ubx3 at endocytic sites. Mechanistically, Ubx3 regulates dynamics and patch lifetime of the early arriving protein Ede1 ~~~
- A Role for the Twins Protein Phosphatase (PP2A-B55) in the Maintenance of Drosophila Genome Integrity...of the pros gene. ku70/irbp was tagged in vitro, and the entire fosmid construct was then inserted into a y stock carrying an attP landing site at 65B using a nanos-FC31 integrase source [Sarov et al. 2016; the ku70MT bearing line was obtained from the Vienna Drosophila Resource Center (VDRC) stock center ~~~