51 Results
for term "sites"
- Substrate Requirements for a Novel Archaeal Endonuclease That Cleaves Within the 5′ External Transcribed Spacer of Sulfolobus acidocaldarius Precursor rRNA...and the central helical domains; a second cleavage (59 to position 231) is in the singlestranded region between the central and the 39 helical domains; and a third cleavage is at the 59 ETS16S junction (59 to position 11). The three sites share a common consensus sequence around the position of cleavage. We have ~~~
- Control of Ribosomal Protein L1 Synthesis in Mesophilic and Thermophilic Archaea...extensively investigated. As in Bacteria, regulation takes place at the level of translation. The regulator protein MvaL1 binds preferentially to its binding site on the 23S rRNA, and, when in excess, binds to the regulatory target site on its mRNA and thus inhibits translation of all three cistrons ~~~
- Expression Vectors for Methanococcus maripaludis: Overexpression of Acetohydroxyacid Synthase and β-Galactosidase...received March 23, 1999 Accepted for publication May 5, 1999 ABSTRACT A series of integrative and shuttle expression vectors was developed for use in Methanococcus maripaludis. The integrative expression vectors contained the Methanococcus voltae histone promoter and multiple cloning sites designed for ef ~~~
- Transcriptional Regulation of Genes Encoding the Selenium-Free [NiFe]-Hydrogenases in the Archaeon Methanococcus voltae Involves Positive and Negative Control Elements...for four such enzymes (Halboth and Klein 1992), two (Zillig et al. 1989). This observation raised interest in of which contain selenocysteine residues as ligands of the analysis of promoter structures in Archaea. Archaeal the Ni atom in their primary reaction sites. The other promoters resemble those ~~~
- Genetic Requirements for the Function of the Archaeal Virus SSV1 in Sulfolobus solfataricus: Construction and Testing of Viral Shuttle Vectors...dlacZDM15) supE44 hsdR17 thi-1 recA1 endA1 deoR Woodcock et al. (1989) gyrA96 relA1 D(lacZYA-argF) U169 Plasmids pBluescript II Cloning plasmids (SK1, KS1) Stratagene pC32 Entire SSV1 genome in BamHI site of pBR325 Yeats et al. (1982) pC322Sal pC32 with Sal I site in pBR325 deleted H. P. Arnold ~~~
- Genetics of Nitrogen Regulation in Methanococcus maripaludis...maripaludis to study nitrogen metabolism and its regulation. We present evidence for a nitrogen regulon in Methanococcus and Methanobacterium species containing genes of nitrogen metabolism that are regulated coordinately at the transcriptional level via a common repressor binding site sequence, or operator ~~~
- Molecular Analysis of pDL10 from Acidianus ambivalens Reveals a Family of Related Plasmids from Extremely Thermophilic and Acidophilic Archaea...volume of 50 ml in the buffer supplied by the manufac-essential for replication and maintenance in the host turer (recognition site: TT9AA). A total of 400 ng of the MseI-cells. digested DNA was incubated with MBN or nuclease S1 in a total volume of 300 ml in the appropriate buffer followed by phenol ~~~
- Genetic Identification of Three ABC Transporters as Essential Elements for Nitrate Respiration in Haloferax volcanii...characterized by H. volcanii has a restriction system that recognizes GATCmethylated DNA, and consequently the transformation fre-several well-conserved sequence motifs: a Walker site A quency using unmethylated DNA is 1000-fold higher than that(GXXGXGKS/T; X represents any amino acid), a heliusing dam ~~~
- Isolation of Acetate Auxotrophs of the Methane-Producing Archaeon Methanococcus maripaludis by Random Insertional Mutagenesis...-plex reduces CO2 to the oxidation level of CO and nome at the site of the cloned genomic DNA (Sandbeckcombines it with HS-CoA and the methyl group to form and Leigh 1991). If the cloned fragment is internal toacetyl-CoA. In M. maripaludis, the biosynthetic activity a gene or operon, integration will disrupt ~~~
- Divergence of the Hyperthermophilic Archaea Pyrococcus furiosus and P. horikoshii Inferred From Complete Genomic Sequences..., cys, thr, and mal operons. P. horikoshii is auxotrophic for tryptophan and histidine and is unable to utilize maltose, unlike P. furiosus. In addition, the genomes differ considerably in gene order, displaying displacements and inversions. Six allelic intein sites are common to both Pyrococcus genomes ~~~