16 Results
for term "sites"
- Aberrant Splicing of an Alternative Exon in the Drosophila Troponin-T Gene Affects Flight Muscle Development...replaces the exon 10b isoform. Functional differences between the 10a and 10b TnT isoforms may be due to different potential phosphorylation sites, none of which correspond to known phosphorylation sites in human cardiac TnT. The absence of TnT mRNA in up1 IFM reduces mRNA levels of an IFM ~~~
- Regulation of Axon Guidance by Slit and Netrin Signaling in the Drosophila Ventral Nerve Cord...site of the sixth intron (Rajagopalan et al. 2000). For the analysis of fra, we used fra3 and fraGA957. The molecular natureof the fra3mutation is a tryptophan to stop codon change at amino acid position 1028 (W1028Stop). The nature of the mutation in fraGA957 is not known. For the analysis of abelson ~~~
- A Gain-of-Function Screen for Genes That Influence Axon Guidance Identifies the NF-κB Protein Dorsal and Reveals a Requirement for the Kinase Pelle in Drosophila Photoreceptor Axon Targeting...the utility of our screen. The genes we identified encode proteins of diverse classes, some acting near the cell surface and others in the cytoplasm or nucleus. We found that one GS line drove misexpression of the NF-kB transcription factor Dorsal, causing motor axons to bypass their correct termination sites ~~~
- A Clonal Genetic Screen for Mutants Causing Defects in Larval Tracheal Morphogenesis in Drosophila...on the left arm of the second chromosome as the other chromosomes were screened by a similar approach by others (M. Krasnow, personal communication; Ghabrial and Krasnow 2006; Levi et al. 2006). 2280 M. M. Baer, A. Bilstein and M. Leptin We therefore used a chromosome carrying the FRT recombination site, FRT ~~~
- Rhythm Defects Caused by Newly Engineered Null Mutations in Drosophila's cryptochrome Gene...called period (per). The seminal cryb mutation was thus recognized because it eliminated the normal daily cycles of luminescence mediated by a perluc transgene in real-time monitorings of whole flies (Stanewsky et al. 1998). This mutation entails an amino-acid substitution within the flys CRY at a site ~~~
- Bedraggled, a Putative Transporter, Influences the Tissue Polarity Complex During the R3/R4 Fate Decision in the Drosophila Eye..., the predicted allele. Generation of UAS-bdg transgenic flies: The CG8291-RB (bdg) transcript was amplified by PCR from the full-length cDNA clone, SD06851 (Research Genetics), using 59 GAC AAATCAGCTGCGACATC and 39 AAGCAAGCGGATATGTG GAT primers. The 59 and 39 primer included BglII and NotI sites ~~~
- Temporal Control of Cell-Specific Transgene Expression in Caenorhabditis elegans...-activated site-specific recombination in mice. Proc. Natl. Acad. Sci. USA 93: 1088710890. Fire, A., S. W. Harrison and D. Dixon, 1990 A modular set of lacZ fusion vectors for studying gene expression in Caenorhabditis elegans. Gene 93: 189198. Fujiwara, M., T. Ishihara and I. Katsura, 1999 A novel WD40 protein ~~~
- Caenorhabditis elegans SDF-9 Enhances Insulin/Insulin-Like Signaling Through Interaction With DAF-2...Laboratories, 2185 East Mall, University of British Columbia, Vancouver, BC V6T 1Z4, Canada. E-mail: driddle@interchange.ubc.ca Genetics 177: 661666 (September 2007) transposons of the Tc family insert into or near genes preferentially at consensus sites (Collins et al. 1987). We usedmut-2 in a screen ~~~
- The flare Gene, Which Encodes the AIP1 Protein of Drosophila, Functions to Regulate F-Actin Disassembly in Pupal Epidermal Cells...of the actin and microtubule cytoskeletons and disruption of these can at least partially phenocopy inturned mutations (Turnerand Adler 1998). The activity of the PCP and PCP effector proteins likely results in one or more proteins localizing at and marking the site for hair initiation. Once this site ~~~
- The Bro1-Domain Protein, EGO-2, Promotes Notch Signaling in Caenorhabditis elegans...at a series of polymorphic sites (Figure 1A). Genomic DNA from each recombinant line was amplified (at 95 for 1 min; 30 cycles of 94 for 30 sec, 55 for 30 sec, 70 for 2 min; 70 for 5 min), digested with an appropriate restriction enzyme, and analyzed by agarose gel electrophoresis to determine whether it had ~~~