299 Results
for term "sites"
- Feeding-Related Traits Are Affected by Dosage of the foraging Gene in Drosophila melanogaster...-dependent relationship between the foraging gene and its pleiotropic inuence on these feeding-related traits. Our analysis of foragings transcription start sites, termination sites, and splicing patterns using rapid amplication of cDNA ends (RACE) and full-length cDNA sequencing, revealed four independent promoters ~~~
- H2O2-Sensitive Isoforms of Drosophila melanogaster TRPA1 Act in Bitter-Sensing Gustatory Neurons to Promote Avoidance of UV During Egg-Laying..., whether such UV sensitivity served any physiological purposes in animal behavior was unclear. Here, we demonstrate that H2O2-sensitive dTRPA1 isoforms promote avoidance of UV when adult Drosophila females are selecting sites for egglaying. First, we show that blind/visionless females are still capable ~~~
- A Zebrafish Model for a Human Myopathy Associated with Mutation of the Unconventional Myosin MYO18B...repeats (CRISPR)/Cas9 mutagenesis: A target sequence in exon 3 of the myo18b gene (59-GGCCGAGATGTCGCTGCGAG-39) was identied using ZiFiT Targeter software (http://zit.partners. org/ZiFiT/) and the sequence inserted into the BsaI site of pDR274 guide RNA (gRNA) expression vector (Hwang et al. 2013 ~~~
- Human SOD1 ALS Mutations in a Drosophila Knock-In Model Cause Severe Phenotypes and Reveal Dosage-Sensitive Gain- and Loss-of-Function Components..., and dsodG85R using previously reported methods (Staber et al. 2011). Briey, we used the ends-out targeting vector p[w25.2] that contains the mini whitemarker (white+), a selectable red eye color, anked by LoxP sites for subsequent removal by Cre recombinase (Figure 1). Homology arm 1, corresponding ~~~
- The Caenorhabditis elegans NF2/Merlin Molecule NFM-1 Nonautonomously Regulates Neuroblast Migration and Interacts Genetically with the Guidance Cue SLT-1/Slit...the conserved FERMdomain regions, and the ok754 in-framedeletion removes the entire FERM C domain, including the putative actin-binding site (Figure 2B). RNAi ofnfm-1 caused embryonic lethality (Skop et al. 2004). Thus, lq132 is likely a hypomorphic mutation and retains some function. ok754 mutants have wild ~~~
- Extrinsic Repair of Injured Dendrites as a Paradigm for Regeneration by Fusion in Caenorhabditis elegans...in the regeneration process required for bypassing the lesion site. Failure of reconnection to the severed dendrites results in degeneration of the distal end of the neuron. We discover pruning of excess branches via EFF-1 that acts to recover the original wild-type arborization pattern in a late stage of the process ~~~
- Functional Equivalence of the SOX2 and SOX3 Transcription Factors in the Developing Mouse Brain and Testes...genes with overlapping and unique sites of expression during embryogenesis. It is currently unclear whether SOX2 and SOX3 have identical or different functions. Here, we use CRISPR/Cas9-assisted mutagenesis to perform a gene-swap, replacing the Sox3 ORF with the Sox2 ORF to investigate their functional ~~~
- Pleiotropic Effects of Loss of the Dα1 Subunit in Drosophila melanogaster: Implications for Insecticide Resistance...to particular ligands and their permeability to particular cations. Individual subunits consist of a pore-forming transmembrane domain coupled to a large extracellular N-terminal domain, which forms the endogenous ligand-binding site with adjacent subunits (Corringer et al. 2000). Upon ACh binding ~~~
- julius seizure, a Drosophila Mutant, Defines a Neuronal Population Underlying Epileptogenesis...2017 1261 (14.7%) between sdaiso7.8 and the transposon insertion line P[GawB]386Y, which has an insertion site near the gene amon at polytene bands 97C497C5 and sdaiso7.8 complementedmolecularly dened deciencies from the aminopeptidase N locus. This suggested that the sda locus had been mislocalized ~~~
- Sequential, Divergent, and Cooperative Requirements of Foxl2a and Foxl2b in Ovary Development and Maintenance of Zebrafish...design and assembly for foxl2a and foxl2b The rst exon sequences of foxl2a and foxl2b genomic loci from the Ensembl zebrash genome browser were veried by PCR and sequencing. Potential TALEN target sites in the loci were searched using the TAL Effector Nucleotide Targeter (https ~~~