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  • Actin and Endocytosis in Budding Yeast
    Bruce L. Goode, Julian A. Eskin, Beverly Wendland
    Genetics February 2015 199: 315-358; https://doi.org/10.1534/genetics.112.145540
    ...roles played by actin polymerization at these sites, while providing a historical perspective of how the landscape has changed since the preceding version of the YeastBookwas published 17 years ago (1997). Finally, we discuss the key unresolved issues and where future studies might be headed. KEYWORDS S ~~~
  • Autophagic Processes in Yeast: Mechanism, Machinery and Regulation
    Fulvio Reggiori, Daniel J. Klionsky
    Genetics June 2013 194: 341-361; https://doi.org/10.1534/genetics.112.149013
    ..., cells have an array of processes for breaking down proteins and other macromolecules, as well as organelles, and each of these distinct processes differ with regard to the machinery involved, the nature of the substrate, and the site of sequestration. The two primary mechanisms for subcellular ~~~
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    Autophagic Processes in Yeast: Mechanism, Machinery and Regulation
    Fulvio Reggiori, Daniel J. Klionsky
    Genetics Jun 2013, 194 (2) 341-361; DOI: 10.1534/genetics.112.149013
    Figure 8
    Figure 8
    Mechanism of cargo recruitment during the Cvt pathway. Shortly after synthesis, prApe1 forms dodecamers that subsequently self-assemble in a larger oligomer that has been called the prApe1 complex. Association with the Atg19 autophagy receptor and oligomers of Ams1 (and additional cargo proteins) leads to the generation of the Cvt complex. The subsequent interaction between Atg19 and the autophagy adaptor Atg11 allows the movement of the Cvt complex within proximity of the vacuole through a mechanism that requires actin filaments and the Arp2/3 complex. This relocalization, which probably also coordinates the trafficking of Atg9-positive membranes, participates in the formation of the PAS. At this site, the interaction between Atg19 and Atg8 plays a key role in the sequestration of the Cvt complex into Cvt vesicles. One of the primary differences between selective and nonselective macroautophagy is that the sequestering vesicles of the former exclude bulk cytoplasm and contain primarily the targeted cargo. The electron micrographs depict the electron dense Cvt complex detected with antiserum to Ape1 (left) and a phagophore sequestering a Cvt complex marked with an antibody that detects GFP–Atg8 (right). The electron micrographs in this figure were modified from data previously published in Yen et al. (2010) and are reproduced by permission of the American Society for Cell Biology, copyright 2010.
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