6 Results
for term "sites"
- Cell Biology of the Caenorhabditis elegans Nucleus...) or a single X chromosome (in males). The functional organization of a C. elegans chromosome is somewhat different from that of chromosomes in vertebrate cells. First, while in vertebrate cells chromosomes typically have a single centromere (the site of kinetochore assembly, through which chromosomes associate ~~~
- The Natural Biotic Environment of Caenorhabditis elegans...environment), followed by microbiome analysis. Substrate samples were also assessed as controls for many of the isolated individuals. In spite of the differences in processing protocols and collection sites of the samples from France/Portugal and those from Germany, the analysis revealed signicant ~~~
- Programmed Cell Death During Caenorhabditis elegans Development...subunit of the CED-3 caspase and is not expected to act as a functional caspase. Although CSP-2 has an overall sequence similarity to the protease domain of the CED-3 caspase, it lacks the invariant catalytic pentapeptide QACXG (C is the active site and X could be R, Q, or G) that is found in all active ~~~
- Next-Generation Sequencing-Based Approaches for Mutation Mapping and Identification in Caenorhabditis elegans...) and Uncoordinated (impaired in its motor movements) phenotypes. EMS: ethyl methanesulfonate HA: Hawaiian Indel: insertion/deletion of genetic material LOESS: Local regression NGS: next-generation sequencing ORF: open reading frame RAD: restriction site-associated DNA SNP: single-nucleotide polymorphism SV ~~~
Figure 12Spatial organization of C. elegans chromosomes. Examples of X chromosome (top) and chromosome I (bottom) are shown. Chromosome conformation capture (Hi-C) analysis was carried out on chromosomes from C. elegans embryos. The data from Hi-C analyses are typically depicted on a matrix where all chromosomal loci (in this case binned in 50-kb resolution) are on both the x- and y-axes, and the frequency of interaction (as reflected by the number of reads that span two loci) is color coded, with darker colors indicating a higher incidence of interaction (or more reads). Interactions will obviously be the greatest between two adjacent loci on the same chromosome, generating a very dark diagonal. The panels shown in this figure focus on a few megabases to each side of this diagonal. Diamond-shaped structures, such as the ones seen along the diagonal for the X chromosome, reflect TADs and indicate that there is a higher level of interaction between distant sites within the coordinates of the TAD than outside the TAD. This analysis revealed that C. elegans chromosomes are organized in megabase-sized TADs separated by boundaries (green lines; darker green indicates stronger boundary). The organization in TADs is more pronounced for the X chromosome than for autosomes (chromosome I shown as example). Figure courtesy of Barbara Meyer; data from Crane et al. (2015).