26 Results
for term "sites"
- CRISPR Technology Reveals RAD(51)-ical Mechanisms of Repair in Roundworms: An Educational Primer for Use with “Promotion of Homologous Recombination by SWS-1 in Complex with RAD-51 Paralogs in Caenorhabditis elegans”...is referred to as intersister (IS) repair. Although IS repair cannot generate crossovers, its contributions to meiosis have been well documented (Goldfarb Figure 1 Sites of mitotic and meiotic recombination in C. elegans. The gonads of C. elegans are a syncitium of nuclei, which is organized spatiotemporally ~~~
- Sorting Out Identities: An Educational Primer for Use with “Novel Tools for Genetic Manipulation of Follicle Stem Cells in the Drosophila Ovary Reveal an Integrin-Dependent Transition from Quiescence to Proliferation”...a critical role in determiningwhen, where, and to what level genes are expressed. A promoter is a region of DNA located at or just upstream of thetranscriptionalstartsiteofagene.Thecorepromoter includes the transcriptional start site and denes the direction of transcription. In addition, it can include ~~~
- Genetics on the Fly: A Primer on the Drosophila Model System.... (2014); (13) Fonseca et al. (2013); (14) Guillen et al. (2014); (15) Zhou et al. (2012); and (16) Zhou and Bachtrog (2015). Primer 819 stages, transcription start and stop sites, alternative splicing patterns, promoter and other regulatory elements (and proteins that bind them), histone variants ~~~
- An Ancient Yeast for Young Geneticists: A Primer on the Schizosaccharomyces pombe Model System.... A similar system exists in ssion yeast. The ability to recombine homologous sequences allows researchers to construct strains that carry novel combinations of alleles by (1) the direct introduction of foreign or otherwise modied pieces of DNA to a targeted site in the yeast genome and (2) the introduction ~~~
- Mating and Memory: An Educational Primer for Use with “Epigenetic Control of Learning and Memory in Drosophila by Tip60 HAT Action”..., modications on histone tails can recruit and bind specic protein domains, such as those found in particular families of transcription factors or chromatin-binding proteins. Often both mechanisms work in tandem, whereby distinct histone modications act as docking sites, recruiting other chromatin ~~~
- Learning to Fish with Genetics: A Primer on the Vertebrate Model Danio rerio...et al. 2007). Insertion sites were identied by direct sequencing of genomic DNA of F1 generation adult males carrying heterozygous insertions (Varshney et al. 2013). An important drawback to this approach is that each individual in the screen contains multiple insertion sites. Therefore, multiple ~~~
- Population Genetics and a Study of Speciation Using Next-Generation Sequencing: An Educational Primer for Use with “Patterns of Transcriptome Divergence in the Male Accessory Gland of Two Closely Related Species of Field Crickets”...resources. Andrs et al. use the latest sequencing technologies to systematically identify and characterize sites in the DNA that vary within, and have diverged between, species to explore speciation in crickets. This primer, coupled with that article, will help instructors introduce and reinforce important ~~~
- SMG-ly Knocking Out Gene Expression in Specific Cells: An Educational Primer for Use with “A Novel Strategy for Cell-Autonomous Gene Knockdown in Caenorhabditis elegans Defines a Cell-Specific Function for the G-Protein Subunit GOA-1”...cloned genes were widely available, investigators could use the tools of molecular to delete a gene or knock down its expression in particular tissues. In mice, the most common method uses the site-specic recombination enzyme Cre, which produces targeted crossovers at sequences known as loxP sites ~~~
- Studying Recombination with High-Throughput Sequencing: An Educational Primer for Use with “Fine-Scale Heterogeneity in Crossover Rate in the garnet-scalloped Region of the Drosophila melanogaster X chromosome”...genome. As they discuss, researchers have found that recombination events do not happen randomly across the genome in most organisms studied. Instead, some areas of the genome are far more likely to be the sites of recombination events (crossovers) than other parts of the same genome. Humans have narrow ~~~
- More than Meets the Eye: A Primer for “Timing of Locomotor Recovery from Anoxia Modulated by the white Gene in Drosophila melanogaster”...into various locations in the Drosophila genome, and its expression is determined by the regulatory elements near that insertion site. The UAS regulatory DNA sequence also can be attached to a target gene of interest to control activation of that target gene. When a y bears both a GAL4 driver of expression ~~~