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  • A New Reversion Assay for Measuring All Possible Base Pair Substitutions in Saccharomyces cerevisiae
    Teresa-Marie Williams, Rebecca M. Fabbri, Jason W. Reeves, Gray F. Crouse
    Genetics July 2005 170: 1423-1426; https://doi.org/10.1534/genetics.105.042697
    ...the development of a sequence is that this codon is part of a TaqI restrictionseries of strains that together can detect any possible site, which is destroyed by any change in positions 1base pair substitution in the Glu-50 residue of TRP5. or 2 of the codon. Thus any mutation in nucleotideEach strain reverts ~~~
  • Evolutionary History of Silene latifolia Sex Chromosomes Revealed by Genetic Mapping of Four Genes
    Dmitry A. Filatov
    Genetics June 2005 170: 975-979; https://doi.org/10.1534/genetics.104.037069
    ..., according to the map reported by Nicolas et al. (2005), the SlX1male. As none of these polymorphisms were located at restriction sites, the segregation of the SlssX gene was gene is the closest to the pseudoautosomal region, which is also consistent with the evolutionary stratatested by direct sequencing ~~~
  • Mutations in Snail Family Genes Enhance Craniosynostosis of Twist1 Haplo-insufficient Mice
    Kathleen F. Oram, Thomas Gridley
    Genetics June 2005 170: 971-974; https://doi.org/10.1534/genetics.105.041277
    .... Nat. Rev. Mol. Cell Biol. 3: 155166. Bourgeois, P., A. L. Bolcato-Bellemin, J. M. Danse, A. Bloch- Opperman, L. A., 2000 Cranial sutures as intramembranous bone Zupan, K. Yoshiba et al., 1998 The variable expressivity and growth sites. Dev. Dyn. 219: 472485. incomplete penetrance of the Twist ~~~
  • Comparison of the X and Y Chromosome Organization in Silene latifolia
    Jitka Zluvova, Bohuslav Janousek, Ioan Negrutiu, Boris Vyskot
    Genetics July 2005 170: 1431-1434; https://doi.org/10.1534/genetics.105.040444
    ...chromosome deletion lines used), we conclude that a part of the Y chromosome (covering a region corresponding to at least 23.9 cM on the X chromosome) has been inverted. The gradient in silent-site divergence suggests that this inversion took place after the recombination arrest in this region. Because ~~~
  • A New Reversion Assay for Measuring All Possible Base Pair Substitutions in Saccharomyces cerevisiae
    Teresa-Marie Williams, Rebecca M. Fabbri, Jason W. Reeves, Gray F. Crouse
    Genetics Jul 2005, 170 (3) 1423-1426; DOI: 10.1534/genetics.105.042697
    F<span class="sc">igure</span> 1.—
    Figure 1.—
    (A) Alignment of the most conserved region of the S. cerevisiae TRP5 protein with various fungal and bacterial homologs. Alignment was done using CLUSTAL W (1.74) multiple sequence alignment (Thompson et al. 1994). The shaded residues are similar, and those in yellow are identical in all species compared. The arrow marks the Glu-50 residue chosen for mutagenesis in this study. (B) Sequence surrounding the Glu-50 codon (GAA) of Trp5. The TRP5 wild-type sequence has a TaqI site overlapping the Glu-50 codon, but replacement of the base at nucleotide position 148 or 149 by any base other than wild type (N) destroys the restriction site, as well as renders the strain Trp−.
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