40 Results
for term "sites"
- A High Copy Suppressor Screen for Autophagy Defects in Saccharomyces arl1Δ and ypt6Δ Strains...proteins, but also requires transport of membrane vesicles to the phagophore assembly site (PAS). In yeast, many small GTP-binding proteins, membrane trafc regulators, are essential fordifferent stagesofautophagy, fromthe formation of the autophagosome to the fusion of the autophagosome and the vacuole ~~~
- Genome-Wide Screen for Haploinsufficient Cell Size Genes in the Opportunistic Yeast Candida albicans.... M. Wang, and C. R. Li, 2016 Sec15 links bud site selection to polarised cell growth and exocytosis in Candida albicans. Sci. Rep. 6: 26464. Harvey, S. L., and D. R. Kellogg, 2003 Conservation of mechanisms controlling entry into mitosis: budding yeast wee1 delays entry into mitosis and is required ~~~
- Mapping the Synthetic Dosage Lethality Network of CDK1/CDC28...strongly depends on the cell cycle (Howell and Lew 2012). Bem1 is an adaptor protein that, together with the small GTPase Bud1, is important for bud site selection and bud growth (Bender and Pringle 1991). Bud2 is a GTPaseactivating protein (GAP) for Bud1 (Benton et al. 1993; Cvrckova and Nasmyth 1993 ~~~
- Sleeping Beauty Transposon Mutagenesis as a Tool for Gene Discovery in the NOD Mouse Model of Type 1 Diabetes...to generate articial alleles on the NOD genetic background. The SB transposon can insert within genes and disrupt transcript expression (Horie et al. 2003; Carlson et al. 2003; Ivics et al. 1997). Its unique sequence also serves as a molecular tag to rapidly identify the site of insertion without ~~~
- CRISPR/Cas9-Mediated Genome Editing and Mutagenesis of EcChi4 in Exopalaemon carinicauda...chitinase 4 (EcChi4), shrimps with indel mutations were obtained. Further analysis showed that the mutations could be transmitted to the next generation. This is the rst time that site-specic genome editing has been successfully demonstrated in a decapod, and will further contribute to the study ~~~
- Mutations that Separate the Functions of the Proofreading Subunit of the Escherichia coli Replicase...replication forks. To explore this model, we generated a pentapeptide insertion mutant library of the dnaQ gene, along with site-directed mutants, and screened for separation of function mutants. We report the identication of separation of function mutants from this screen, showing that proofreading function ~~~
- Identification of Functional Domains in the Cohesin Loader Subunit Scc4 by a Random Insertion/Dominant Negative Screen...and Bates 2010; Lopez-Serra et al. 2014). After loading is completed, ATP is hydrolyzed by the SMC proteins. In turn, cohesin is dissociated from Scc2/Scc4 and is translocated from the loading site (Weitzer et al. 2003; Hu et al. 2011; Ladurner et al. 2014; Murayama and Uhlmann 2015). Some evidence suggests ~~~
- Phenotypes Associated with Second Chromosome P Element Insertions in Drosophila melanogaster...misleading symbols in the Bloomington Drosophila Stock Center collection to determine whether the insertions are responsible for recessive lethal or sterile phenotypes. MATERIALS AND METHODS Our approach was straightforward: we identied chromosomal deletions that encompass the insertion sites of P elements ~~~
- Identification of Genes in Saccharomyces cerevisiae that Are Haploinsufficient for Overcoming Amino Acid Starvation...to identify SMM-sensitive strains (Swanson et al. 2003; Kim et al. 2005; Zhang et al. 2008), some genes may not have been identied due to second-site suppressors that may arise (Huang and OShea 2005; Teng et al. 2013; Giaever and Nislow 2014). This would prevent the identication of a true positive ~~~
- A Genetic Mosaic Screen Reveals Ecdysone-Responsive Genes Regulating Drosophila Oogenesis...for GSC maintenance and proliferation (Ables and Drummond-Barbosa 2010). In brief, an FRT site lies proximal to a mutation in a gene of interest in trans to another FRT chromosome arm carrying the corresponding wildtype allele linked to a GFP marker. FLP catalyzes mitotic recombination between the FRT ~~~