334 Results
for term "sites"
- Chromatin Organization and Remodeling of Interstitial Telomeric Sites During Meiosis in the Mongolian Gerbil (Meriones unguiculatus)...sites (ITSs) can also be found along the chromosomes, especially near the centromere, where they may appear following chromosomal rearrangements like Robertsonian translocations. There is no dened role for ITSs, but they are linked to DNA damage-prone sites. We were interested in studying ~~~
- Repair of Meiotic DNA Breaks and Homolog Pairing in Mouse Meiosis Requires a Minichromosome Maintenance (MCM) Paralog...P sites was integrated into an intron (Figure S1A). This allele is referred to asMcmdc2Gt. Founder animals were genotyped by PCR and the expression pattern of Mcmdc2 was analyzed by RT-qPCR and lacZ activity staining (Figure S1B and Figure S2). These analyses showed that Mcmdc2 is expressed primarily ~~~
- Cooperation Between Kinesin Motors Promotes Spindle Symmetry and Chromosome Organization in Oocytes.... To generate a mutation in Klp54D, we excised the P{EP} G7530 transposable element, which is inserted 290 bp downstream of the Klp54D coding sequence. We screened excisions for deletions of Klp54D by PCR after crossing to Df(2R) ED3385. We obtained one deletion of 2.2 kb upstream of the insertion site, which ~~~
- Genetic Contributors to Intergenerational CAG Repeat Instability in Huntington’s Disease Knock-In Mice...transgenic (R/6) models, with similar CAG repeat sizes, but distinct transgene insertion sites (Mangiarini et al. 1997). DNA repair genes Msh2, Msh3, Msh6, and Neil1 have been identied as trans-acting modiers of intergenerational repeat instability (Wheeler et al. 2003; Lloret et al. 2006; Mllersen et al ~~~
- A Pooled Sequencing Approach Identifies a Candidate Meiotic Driver in Drosophila...of the lysate was then processed following the manufacturers instructions. Illumina libraries were then generated using the TruSeq Kit, and sequenced on one lane using NextSequation (75 bp single-end reads). Processing sequences and identication of heterozygous sites Sequences were aligned to D. melanogaster ~~~
- Remarkably Long-Tract Gene Conversion Induced by Fragile Site Instability in Saccharomyces cerevisiae...causes breaks at chromosomal locations called common fragile sites. Deletions causing loss of heterozygosity (LOH) in human tumors are strongly correlated with common fragile sites, but the role of gene conversion in LOH at fragile sites in tumors is less well studied. Here, we investigated gene ~~~
- Correlation of Meiotic DSB Formation and Transcription Initiation Around Fission Yeast Recombination Hotspots...as the Atf1 protein, we discuss a possibility that Atf1 promotes DSB formation through inducing transcription from its binding site. Moreover, comparison of previously published genome-wide data sets unveiled proximity of hotspots and meiotically transcribed regions. These observations may suggest that ~~~
- A Slowed Cell Cycle Stabilizes the Budding Yeast Genome...in budding yeast. We were studying a regionof a specic chromosome that previouslywehad shown is associated with high levels of instability (Paek et al. 2009). This region, which we called a fragile site, was immediately next to the ERV14 gene. Unexpectedly, we found that the fragile site sequence does ~~~
- Annealing of Complementary DNA Sequences During Double-Strand Break Repair in Drosophila Is Mediated by the Ortholog of SMARCAL1...downstream of the conserved lysine codon from BACPAC genomic DNA clone library (identier: BACR13M11) and inserted into the pSL1180 vector. The QuickChange Site-Directed Mutagenesis Kit (Agilent Technologies) was used to introduce a mutation in the PAM recognition site as well as two single base pair changes ~~~
- Meiotic Crossing Over in Maize Knob Heterochromatin...of recombination nodules (RNs) that mark sites of crossing over. We found that MLH1 foci occur at similar frequencies per unit length of SC in interstitial knobs and in the 1 mm segments of SC in euchromatin immediately to either side of interstitial knobs. These results indicate not only that crossing over occurs ~~~