2 Results
for term "sites"
- An Unusual Role for doublesex in Sex Determination in the Dipteran Sciara...designed to allow the joining of the four fragments in the order E1I1 I1E2I2I2E3I3I3E4 and this construct was cloned in the pUAST vector. To do this, the primers contained restriction enzyme sites: e1i1-2 and i1e2i1-1 contained the XbaI sequence, i1e2i2-2 and i2e3i3-1 contained the PstI sequence, i2e3i3 ~~~
Figure 7Molecular evolution of the organization and splicing regulation of gene dsx in insects. It follows the phylogeny shown in Figure 6. Exons (E) (boxes) and introns (lines) are not drawn to scale. The number following E indicates the position of the corresponding exon in the molecular organization of the dsx gene. The dotted lines for introns indicate that their lengths remain unknown. Blue exons are common to both sexes, red exons indicate female-specific exons, and green exons represent male-specific exons. AAA stands for poly-A+. TSC stands for translation stop codon. TSC-F and TSC-M stand for translation stop codon in female and male mRNAs, respectively. Black and yellow circles and green squares within exons represent the binding sites for the corresponding specific splicing factors (see main text).