Table 1 Mutants identified in a screen for altered peroxisomally-targeted fluorescence
Gene (accession number)Function of proteinAlleleAliasNucleotide changeProtein or transcript changePhenotypes vs. reference allele
Fatty acid β-oxidation
 SDP1 (At5g04040)Triacylglycerol lipasesdp1-7R330c1075tConserved R359WWeakera
 PXA1 (At4g39850)ATP binding cassette transporterpxa1-4R751g1585aG307E in EAA-like motifSimilar
 ACX1 (At4g16760)Acyl-CoA oxidaseacx1-4R574g1146aConserved G226RSimilar
 ACX2 (At5g65110)Acyl-CoA oxidaseacx2-2R233g603aW154stopSimilar
acx2-3R737g604aConserved G155RSimilar
 MFP2 (At3g06860)2-trans-Enoyl-CoA hydratase and l-3-hydroxyacyl-CoA dehydrogenasemfp2-6R281g3737aConserved G670RSimilara
mfp2-7R778g2736aConserved A454TWeakera
mfp2-8R794/809g2051aConserved G320DSimilara
mfp2-9R1009g2116aConserved E342KSimilara
 PED1 (At2g33150)3-Ketoacyl-CoA thiolaseped1-4R814g928aConserved G109EWeaker
ped1-5R883g1113aConserved G141ESimilar
Indirect fatty acid β-oxidation and glyoxylate cycle
 MLS (At5g03860)Malate synthase (glyoxylate cycle)mls-4R332g1595aIntron 3 splice acceptorSimilar
 ICL (At3g21720)Isocitrate lyase (glyoxylate cycle)icl-3R291g177aConserved E21KWeaker
icl-4R601g366aConserved G84RWeaker
icl-5R951g2128aW371stopSimilar
 PMDH1 (At2g22780)Peroxisomal malate dehydrogenase (fatty acid β-oxidation)pmdh1-2R92c643tQ129stopSimilar
 CHY1 (At5g65940)β-Hydroxyisobutyryl-CoA hydrolase (valine catabolism)chy1-5R189g700aConserved G119DSimilar
chy1-6R499/506g871aConserved D150NSimilar
chy1-7R728g988aConserved G165ESimilar
 MDAR4 (At3g27820)Peroxisomal monodehydroascorbate reductase (hydrogen peroxide detoxification)mdar4-5R340/343g942aConserved G122DSimilar
mdar4-6R541/577g31aConserved G11RSimilar
mdar4-7R923g659aIntron 2 splice acceptorSimilar
Large GFP-PTS1 puncta
 LON2 (At5g47040)Peroxisomal proteaselon2-8R109g650aQ102stopSimilar
lon2-9R498g3610aConserved R537K in arginine finger of AAA+ domainWeaker
lon2-10R973g400aW45stopSimilar
 PXN (At2g39970)Peroxisomal NAD+ and CoA transporterpxn-4R109g2194aIntron 9 splice acceptorSimilar
pxn-5R162c2060tR258stopSimilar
pxn-6R986g1629aIntron 6 splice acceptorSimilar
pxn-7R987/995g2111aConserved G275ESimilar
Miscellanea
 DRP3A (At4g33650)Dynamin-related protein (peroxisome division)drp3a-4R224c755tQ224stopSimilar
drp3a-5R402g281aConserved R94HSimilar
 PME31 (At3g29090)Pectin methylesterasepme31-1R79g701cConserved G141DNot applicable
pme31-2R363g182aConserved G61ENot applicable
pme31-3R922g1023aConserved G201DNot applicable
  • a Comparison to description of previously published alleles.