Table 3 Loss of daf-12 function recapitulates the dauer-associated hyperplasia in the germ line and somatic gonad typical of din-1S(rr94) mutants
GenotypeNo. of germ cells in daueraNo. of proximal somatic gonadal cells in dauera
(A) daf-2(e1370)b34.5 ± 3.5*6 ± 0
(B) din-1S(rr94); daf-2(e1370)b49.7 ± 10.0*9.5 ± 4.6*
(C) daf-2(e1370); daf-12(rh61)b40.4 ± 3.7*6 ± 0
(D) din-1S(rr94); daf-2(e1370); daf-12(rh61)b45.0 ± 9.9*6 ± 0
(E) rrf-1(pk1417); daf-7(e1372)31.8 ± 4.46 ± 0
(F) rrf-1(pk1417); daf-7(e1372);E.V. (RNAi)c30.6 ± 2.9**6 ± 0
(G) rrf-1(pk1417); daf-7(e1372); daf-12(RNAi)40.1 ± 6.7**8.0 ± 6.9
(H) rrf-1(pk1417); daf-2(e1370)d32.5 ± 3.86 ± 0
(I) rrf-1(pk1417); daf-2(e1370); E.V. (RNAi)c,d31.4 ± 2.8***6 ± 0
(J) rrf-1(pk1417); daf-2(e1370); daf-12(RNAi)d37.9 ± 4.0***6.4 ± 1.8
  • * P < 0.0005 (statistical significance) between rows B, C, and D and the control value shown in row A according to a one-tailed t-test with unequal variance. ** P < 0.0005 (statistical significance) between row G and the control value shown in row F according to a one-tailed t-test with unequal variance. *** P < 0.0005 (statistical significance) between row J and the control value shown in row I according to a one-tailed t-test with unequal variance.

  • a Mean ± SD; n ≥ 20.

  • b Animals were synchronized as L1 larvae and shifted to the restrictive temperature (25°) for 48 hr before the cell counts to avoid confounding problems with daf-12 dauer formation. At this point, although all daf-2 animals had formed dauer larvae, the daf-12 were in a transient dauer stage and din-1S animals were still in the L2d stage.

  • c E.V., empty vector was used as an RNAi control.

  • d Full genotype includes qIs56.