Table 5 din-1S cooperates with ILS and the LKB1/AMPK signaling pathway to regulate quiescence in the germline and in the somatic gonad of the dauer larva
GenotypeNo. of germ cells in daueraNo. of proximal somatic gonadal cells in daueraDauer survival (in days)a
(A) daf-2(e1370)b33.6 ± 3.96 ± 033.5 ± 8.3 (207)
(B) din-1S(rr94); daf-2(e1370)b82.0 ± 16.325.4 ± 19.214.4 ± 8.0 (191)
(C) daf-2(e1370) aak-1(tm1944)50.3 ± 7.86 ± 0ND
(D) din-1S(rr94); daf-2(e1370) aak-1(tm1944)b126.9 ± 20.460.3 ± 14.3ND
(E) daf-2(e1370); aak-2(rr48)b76.1 ± 15.26 ± 010.7 ± 2.2 (197)
(F) daf-2(e1370); aak-2(ok524)67.5 ± 9.76 ± 0ND
(G) din-1S(rr94); daf-2(e1370); aak-2(rr48)b,c152.6 ± 28.752.5 ± 25.55.8 ± 1.3 (159)
(H) din-1S(rr94); daf-2(e1370); aak-2(ok524)126.0 ± 24.858.9 ± 23.2ND
(I) daf-2(e1370); par-4(it57)129.8 ± 19.96 ± 0ND
(J) din-1S(rr94); daf-2(e1370); par-4(it57)d211.8 ± 25.975.5 ± 13.2ND
(K) daf-2(e1370) aak-1(tm1944); aak-2(ok524)168.6 ± 39.36 ± 0ND
(L) din-1S(rr94); daf-2(e1370) aak-1(tm1944); aak-2(ok524)d211.5 ± 39.371.9 ± 12.2ND
  • n, sample size. ND, not determined.

  • a Mean ± SD; n ≥ 20 unless otherwise stated.

  • b Full genotype includes qIs56.

  • c din-1S; daf-2; aak-2 mutant animals showed a synthetic dauer recovery phenotype where 14.6% of animals had recovered inappropriately after 72 hr in dauer at 25°. None of the daf-2(e1370), din-1S(rr94); daf-2(e1370), or daf-2(e1370); aak-2(rr48) animals recover prematurely under our culture conditions. n = 300.

  • d n = 15.