Table 1 ecm11 and gmc2 mutants display an excess of Msh4-dependent meiotic crossover events
Genotype (strain)Interval (chromosome)PDTTNPDTotalcMa%WTcM by chrm%WT by chrmNPDobs/NPDexp (±SE)
WT (K842)HIS4-CEN3 (III)344325667526.7 (1.4)100106.0 (III)1000.19 (0.08)
CEN3-MAT (III)427250468120.1 (1.2)1000.25 (0.13)
MAT-RAD18 (III)2554051467436.3 (1.8)1000.22 (0.06)
AD18-HMR (III)395273667422.9 (1.4)1000.30 (0.13)
SPO11-SPO13 (VIII)2514012167339.2 (2.0)10076.3 (VIII)1000.35 (0.08)
SPO13-THR1 (VIII)5659416607.6 (0.8)1000.54 (0.54)
THR1-LYS2 (VIII)296361566229.5 (1.3)1000.11 (0.05)
msh4Δ (K852)HIS4-CEN3 (III)37396147010.9 (1.1)4153.3 (III)500.35 (0.35)
CEN3-MAT (III)4245014755.9 (0.9)291.41 (1.42)
MAT-RAD18 (III)275183746524.2 (1.9)670.55 (0.21)
RAD18-HMR (III)351115046612.3 (1.0)54n.d.
SPO11-SPO13 (VIII)36588345611.6 (1.4)3030.2 (VIII)401.22 (0.71)
SPO13-THR1 (VIII)4232704503.0 (0.6)39n.d.
THR1-LYS2 (VIII)320129245115.6 (1.4)530.34 (0.25)
mlh3Δ (K854)HIS4-CEN3 (III)367157052415.0 (1.0)5662.2 (III)40n.d.
CEN3-MAT (III)415104352211.7 (1.3)581.00 (0.58)
MAT-RAD18 (III)280232451624.8 (1.5)680.20 (0.10)
RAD18-HMR (III)408111051910.7 (0.9)47n.d.
SPO11-SPO13 (VIII)325185651621.4 (1.7)5542.2 (VIII)550.53 (0.22)
SPO13-THR1 (VIII)4754105164.0 (0.6)53n.d.
THR1-LYS2 (VIII)352161251516.8 (1.3)570.25 (0.18)
ecm11Δ (K857)HIS4-CEN3 (III)456371583224.1 (1.1)90134.9 (III)1270.16 (0.07)
CEN3-MAT (III)3974261383630.1 (1.5)1500.29 (0.08)
MAT-RAD18 (III)2604862977542.6 (2.0)1180.34 (0.07)
RAD18-HMR (III)3144532579238.1 (1.9)1660.41 (0.09)
SPO11-SPO13 (VIII)3324413981241.6 (2.2)106116.4 (VIII)1530.73 (0.13)
SPO13-THR1 (VIII)464267873921.3 (1.4)2800.49 (0.18)
THR1-LYS2 (VIII)2104635272553.5 (2.7)1810.60 (0.11)
ecm11[K5R,K101R] (K846)HIS4-CEN3 (III)299316662128.3 (1.5)1060.18 (0.07)
CEN3-MAT (III)3003131062329.9 (1.7)149145.4 (III)1370.31 (0.10)
MAT-RAD18 (III)1743773858951.4 (2.9)1420.53 (0.11)
RAD18-HMR (III)2543241759535.8 (2.1)1560.43 (0.11)
SPO11-SPO13 (VIII)2263253258344.3 (2.7)113122.3 (VIII)1600.77 (0.15)
SPO13-THR1 (VIII)338199053718.5 (1.0)243n.d.
THR1-LYS2 (VIII)1283644653859.5 (3.3)2020.65 (0.11)
gmc2Δ (K906)HIS4-CEN3 (III)218237746230.2 (1.9)1130.27 (0.11)
CEN3-MAT (III)210244946332.2 (2.1)160155.3 (III)1470.32 (0.11)
MAT-RAD18 (III)1362782343747.6 (3.1)1310.45 (0.11)
RAD18-HMR (III)1173101544245.3 (2.5)1980.43 (0.04)
SPO11-SPO13 (VIII)1832491945140.2 (2.8)103120.9 (VIII)1580.61 (0.15)
SPO13-THR1 (VIII)231178741626.4 (2.1)3470.50 (0.19)
THR1-LYS2 (VIII)1032772840854.3 (3.5)1840.55 (0.09)
ecm11Δ msh4Δ (K882)HIS4-CEN3 (III)3585104096.2 (0.8)2349.6 (III)47n.d.
CEN3-MAT (III)3822814114.1 (1.0)204.00 (4.02)
MAT-RAD18 (III)237153539523.2 (2.0)640.48 (0.22)
RAD18-HMR (III)292105440116.1 (1.8)700.95 (0.48)
SPO11-SPO13 (VIII)3385603947.1 (0.9)1836.5 (VIII)48n.d.
SPO13-THR1 (VIII)3403503754.7 (0.8)62n.d.
THR1-LYS2 (VIII)2441181137324.7 (2.8)841.82 (0.58)
ecm11Δ mlh3Δ (K888)HIS4-CEN3 (III)343109445614.6 (1.6)5579.5 (III)751.02 (0.52)
CEN3-MAT (III)330108244013.6 (1.4)680.50 (0.36)
MAT-RAD18 (III)2071661438732.2 (2.9)891.06 (0.30)
RAD18-HMR (III)268129440119.1 (1.8)830.59 (0.30)
SPO11-SPO13 (VIII)272148842822.9 (2.2)5868.9 (VIII)900.93 (0.34)
SPO13-THR1 (VIII)3245613818.1 (1.2)1070.87 (0.88)
THR1-LYS2 (VIII)1681791636337.9 (3.2)1280.89 (0.24)
  • Map distances and interference values were calculated using tetrad analysis and coefficient of coincidence measurements as described previously (Voelkel-Meiman et al. 2015). Four-spore viable tetrads with no more than two gene conversion (non-2:2) events were included in calculations, although cases where adjacent loci display non-2:2 segregation were considered a single (co-conversion) event. See Table S1 for gene conversion frequencies. Table indicates the number of tetratype (TT), parental ditype (PD) and nonparental ditype (NPD) tetrads scored, map distances (in centimorgans; cM) and their corresponding percentages of the wild-type values for individual intervals, and the map distances and the corresponding percentage of wild type for the entire chromosome (chrm) by summing the intervals on III or VIII. The table also indicates the ratio of observed (obs) to expected (exp) NPD tetrads. The number of chromatids III participating in crossover recombination indicates a general increase in interhomolog events in ecm11 mutants relative to wild type: In wild-type four-spore viable tetrads (n = 512), all of the crossover events on chromosome III in a given tetrad involved two chromatids 45% of the time, three chromatids 26% of the time, and four chromatids 27% of the time. In four-spore viable tetrads from ecm11 mutants (n = 878), all of the crossover events on chromosome III in a given tetrad involved two chromatids only 27% of the time, three chromatids 33% of the time, and four chromatids 37% of the time. For the intervals marked with n.d., interference measurements are not obtainable using the coefficient of coincidence method due to an absence of NPD tetrads.

  • a ±SE