Table 2 zip1-N1 expressing meiotic cells display the same excess of Msh4-dependent interhomolog crossovers observed in ecm11 mutants
Genotype (strain)Interval (chromosome)PDTTNPDTotalcM (±SE)%WTcM by chrm%WT by chrmNPDobs/NPDexp (±SE)
WT (YT131)HIS4-CEN3 (III)257231849628.1 (1.9)1000.38 (0.14)
CEN3-MAT (III)340155349817.4 (1.4)100107.4 (III)1000.39 (0.22)
MAT-RAD18 (III)1872881649139.1 (2.4)1000.38 (0.11)
RAD18-HMR (III)295196549622.8 (1.7)1000.37 (0.17)
SPO11-SPO13 (VIII)219260648530.5 (1.7)1000.20 (0.08)
iTHR1-iLEU2 (XI)4039004939.1 (0.9)100n.d.
msh4Δ (AM3313)HIS4-CEN3 (III)5219036148.8 (1.1)311.64 (0.95)
CEN3-MAT (III)5269036198.7 (1.1)5059.2 (III)551.65 (0.96)
MAT-RAD18 (III)3622301260425 (1.9)640.79 (0.24)
RAD18-HMR (III)441162761016.7 (1.5)731.1 (0.41)
SPO11-SPO13 (VIII)465129259611.8 (1.1)390.49 (0.35)
iTHR1-iLEU2 (XI)5873306202.7 (0.5)30n.d.
ecm11Δ (AM3378)HIS4-CEN3 (III)158217437931.8 (1.9)1130.14 (0.07)
CEN3-MAT (III)159215437831.6 (1.9)182151.3 (III)1410.14 (0.17)
MAT-RAD18 (III)1012322135450.6 (3.5)1290.43 (0.12)
RAD18-HMR (III)128229736437.2 (2.3)1630.17 (0.07)
SPO11-SPO13 (VIII)1152242036247.9 (3.5)1570.50 (0.13)
zip1-N1 (SYC123)HIS4-CEN3 (III)2653031258032.2 (1.9)1150.35 (0.11)
CEN3-MAT (III)2093751760139.7 (2.1)228147.9 (III)1380.26 (0.07)
MAT-RAD18 (III)2153551758738.9 (2.1)1000.30 (0.08)
RAD18-HMR (III)2423291858937.1 (2.2)1630.42 (0.11)
SPO11-SPO13 (VIII)1443914057554.9 (2.9)1810.56 (0.08)
iTHR1-iLEU2 (XI)417164558616.6 (1.4)1840.70 (0.32)
zip1-N1 ecm11Δ (SYC142)HIS4-CEN3 (III)2904591276134.9 (1.5)1250.17 (0.05)
CEN3-MAT (III)2624981877839.0 (1.7)224147.1 (III)1370.19 (0.05)
MAT-RAD18 (III)3024282175136.9 (1.9)940.36 (0.09)
RAD18-HMR (III)3074272075436.3 (1.8)1590.35 (0.08)
SPO11-SPO13 (VIII)1985015074953.5 (2.5)1750.43 (0.07)
iTHR1-iLEU2 (XI)432313875324.0 (1.4)2640.34 (0.12)
zip1-N1 msh4Δ (SYC151)HIS4-CEN3 (III)481116259910.7 (1.1)380.62 (0.44)
CEN3-MAT (III)496109260710.0 (1.0)57147.1 (III)540.73 (0.52)
MAT-RAD18 (III)407185659818.5 (1.5)470.65 (0.27)
RAD18-HMR (III)397199460018.6 (1.3)820.37 (0.19)
SPO11-SPO13 (VIII)437138257713.0 (1.1)430.40 (0.29)
iTHR1-iLEU2 (XI)5037305766.3 (0.7)69n.d.
zip1-N1 mlh3Δ (SYC133)HIS4-CEN3 (III)312157647520.3 (1.8)720.70 (0.29)
CEN3-MAT (III)2742051249128.2 (2.2)162110.5 (III)1030.77 (0.23)
MAT-RAD18 (III)2462261348531.3 (2.3)800.63 (0.19)
RAD18-HMR (III)2532211348730.7 (2.3)1350.68 (0.20)
SPO11-SPO13 (VIII)2152362047137.8 (2.8)1240.82 (0.20)
iTHR1-iLEU2 (XI)371103247612.1 (1.3)1330.61 (0.43)
  • Map distances and interference values were calculated using tetrad analysis as described previously (Voelkel-Meiman et al. 2015). Four-spore viable tetrads with no more than two gene conversion (non-2:2) events were included in calculations, although cases where adjacent loci display non-2:2 segregation were considered a single (co-conversion) event. See Table S1 for gene conversion frequencies. Table indicates the number of tetratype (TT), parental ditype (PD) and nonparental ditype (NPD) tetrads scored, map distances (in centimorgans; cM) and their corresponding percentages of wild-type values for individual intervals, and map distances and corresponding percentage of wild type for the entire chromosome (chrm) III (by summing the intervals on III). The table also indicates the ratio of observed (obs) to expected (exp) NPD tetrads. For the intervals marked with n.d., interference measurements are not obtainable due to an absence of NPD tetrads. Data for wild-type and msh4 strains were previously reported (Voelkel-Meiman et al. 2015).