Table 1 Genetic analysis of rad-26
GenotypeaRNAib% Muvcnd
1. Wild typeeNA01702
2. rad-26(tm1991)eNA0.11424
3. lin-1(e1275)eNA412271
4. lin-1(e1275) rad-26(tm1991)eNA891238
5. Wild typeNA01002
6. rad-26(tm1991)NA0308
7. lin-1(n1790)NA8840
8. lin-1(n1790) rad-26(tm1991)NA291592
9. rad-26(tm1991); set-6(ok2195)NA02345
10. egl-27(we3); rad-26(tm1991)NA01678
11. mpk-1(oz140); rad-26(tm1991)NA01596
12. rad-26(tm1911); lin15A(n767)NA01843
13. rad-26(tm1911); lin-15B(n744)NA01688
14. Wild typeControl01556
15. Wild typerad-2601787
16. let-60(n1046)eControl16375
17. let-60(n1046)erad-2685932
18. lin-1(n1790)Control3153
19. lin-1(n1790)rad-2611262
  • a To facilitate comparisons, the following experiments (indicated by line numbers) were conducted in parallel: 1–4, 5–8, 14–15, 16–17, and 18–19. The difference between the percentage of the lin-1(n1790) Muv phenotype in line 7 and line 18 might reflect day-to-day variability or the difference between control RNAi bacteria and standard OP50 bacteria as a food source.

  • b L4 hermaphrodites were fed E. coli containing a control plasmid or a plasmid that expresses dsRNA from the rad-26 gene, and the vulval phenotype of the progeny that grew on these dishes was scored. NA, not applicable.

  • c Adult hermaphrodites were scored as Muv if they displayed one or more ventral protrusions displaced from the site of the vulva. Hermaphrodites were propagated at 20° unless otherwise indicated. The χ2 test was used to compare two values. The following comparisons (indicated by line numbers) were significantly different (P < 0.05): 1 and 3, 2 and 4, 3 and 4, 5 and 7, 6 and 8, 7 and 8, 16 and 17, 18 and 19.

  • d n, number of hermaphrodites examined.

  • e Hermaphrodites were propagated at 15° because lin-1(e1275) and let-60(n1046) cause heat-sensitive Muv phenotypes.