Table 3 Phenotype analysis of histone methylation and SSN8 mutant yeast
Histone targetGenotypeSSN8Unipolar budsElongated budsInvasive growthFlocculation
Wild type+
Δ+++
+, ssn3Δ++++
H3 Lys4set1Δ++
Δ++
+, ssn3Δ+
jhd2Δ++++
Δ++++++++
+, ssn3Δ++++++++
H3 Lys36set2Δ+++++
Δ+++
jhd1Δ++
Δ+++
gis1Δ++++
Δ+++++
rph1Δ+++
Δ+++
H3 Lys79dot1Δ+++
Δ+++
H4 Lys5, -8, -12set5Δ++
Δ++++
  • Phenotypes were scored from yeast harboring the indicated deletions cultured in rich media to mid-logarithmic phase. Yeast were scored as follows: unipolar buds: “−” < 30%; “+” = 30–80%; “++” ≥ 80%; elongated buds, median length-to-width values <1.3 = “−”, ≥ 1.3–1.4 = “+”, ≥ 1.4 = “++” (Table S1). Invasive growth and flocculation scores are based on data in Figure 1, C and D