Table 1 Tandem mass spectrometry analysis of RP-HPLC fractions in C. adamanteus
FractionProtein name% TSCSeq coverage (%)
1aNone
1bLAAO27.9227.7
SVMPII-2b15.5616.2
MYO-1a/b10.0758.3
SVMPIII-57.3230.8
PLA2-1a5.0347.5
PDE4.816.0
SVMPII-1b/d4.8115.6
SVMPIII-2b4.5815.2
2MYO-1b100.0052.1
3SVMPIII-2b100.0018.3
4SVMPIII-2b100.0022.5
5SVMPIII-2b100.0016.1
6None
7CRISP96.2379.2
8SVSP-580.6430.8
CRISP19.3617.6
9None
10PLA2-1a/b100.0091.8
11SVSP-286.3925.7
SVSP-513.6117.5
12SVSP-498.7759.0
13SVSP-776.0855.1
SVSP-423.9231.1
14CTL-1068.0269.2
SVSP-420.6238.5
CTL-11a/b/c7.2852.3
15aCTL-1042.0258.1
CTL-939.2161.3
SVSP-411.4038.5
15bCTL-1027.63100.0
CTL-13f21.4896.0
SVSP-77.3389.0
LAAO7.1670.7
SVSP-46.6585.2
CTL-11a/b5.0299.2
16LAAO45.2139.8
SVSP-725.9255.1
SVSP-420.2745.1
CTL-11a/b/c4.6732.8
17SVSP-449.6845.1
SVSP-725.9842.9
NUC16.986.6
HYAL5.7010.7
18SVMPIII-4d/e98.0129.9
19SVMPIII-380.8643.6
CTL-11a12.8463.3
20aSVMPIII-2b62.6535.9
SVMPII-2c/d17.9930.8
SVMPII-2b17.4730.8
20bSVMPII-2b17.3852.8
LAAO12.6687.6
CTL-108.07100.0
CTL-11a/b5.3699.2
SVMPIII-2b4.9646.9
CTL-13f4.75100.0
SVSP-44.5196.3
20cLAAO17.2476.7
SVMPII-2b9.4042.1
CTL-11a/b7.0399.2
SVMPII-1b/d6.7343.3
CTL-105.0994.0
21SVMPII-1a/b/c/d94.7846.9
  • Toxin identifications based on peptide spectral evidence of isolated peaks from RP-HPLC. CTL, C-type lectin; CRISP, cysteine-rich secretory protein; HYAL, hyaluronidase; LAAO, l-amino-acid oxidase; MYO, myotoxin; NUC, nucleotidase; PDE, phosphodiesterase; PLA2, phospholipase A2; SVMP, snake venom metalloproteinase (types II and III); SVSP, snake venom serine proteinase; TSC = total spectrum count.